Construct: ORF TRCN0000468462
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002262.1_s317c1
- Derived from:
- ccsbBroadEn_05083
- DNA Barcode:
- ACAGGGTCTATCTTCCCTAACTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VKORC1L1 (154807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468462
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154807 | VKORC1L1 | vitamin K epoxide reductase... | NM_173517.6 | 100% | 100% | |
| 2 | human | 154807 | VKORC1L1 | vitamin K epoxide reductase... | XM_011515831.3 | 75.3% | 59.6% | (many diffs) |
| 3 | human | 154807 | VKORC1L1 | vitamin K epoxide reductase... | NM_001284342.3 | 65.2% | 42.8% | 193_194ins110;419_531del |
| 4 | mouse | 69568 | Vkorc1l1 | vitamin K epoxide reductase... | NM_027121.4 | 92% | 97.1% | (many diffs) |
| 5 | mouse | 69568 | Vkorc1l1 | vitamin K epoxide reductase... | NM_001286382.1 | 66.8% | 71% | (many diffs) |
| 6 | mouse | 69568 | Vkorc1l1 | vitamin K epoxide reductase... | NM_001001327.2 | 65.5% | 34.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctcccgtc ctgctaagag tgtcggtgcc gcggtgggag cgggtggccc 121 ggtatgcagt gtgcgctgcc ggaatcctgc tctccatcta cgcctaccac gtggagcggg 181 agaaggagcg ggaccccgag caccgggccc tctgcgacct ggggccctgg gtgaagtgcT 241 CCGCCGCCCT TGCCTCCAGA TGGGGTCGAG GATTTGGTCT TTTGGGTTCC ATTTTTGGAA 301 AGGATGGTGT ATTAAACCAG CCAAACAGTG TCTTTGGACT TATATTTTAT ATACTACAGT 361 TATTACTTGG CATGACAGCA AGCGCTGTGG CGGCTTTGAT CCTCATGACG TCCTCCATCA 421 TGTCGGTCGT GGGGTCCCTG TACCTGGCCT ACATTCTGTA CTTTGTGCTG AAGGAGTTCT 481 GCATCATCTG CATCGTCACG TACGTGCTGA ACTTCCTTCT TCTCATTATC AACTACAAAC 541 GACTAGTTTA CTTGAACGAG GCCTGGAAGC GGCAGCTGCA ACCCAAGCAG GACTGCCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAA CAGGGTCTAT CTTCCCTAAC TAGACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att