Transcript: Mouse NM_027121.4

Mus musculus vitamin K epoxide reductase complex, subunit 1-like 1 (Vkorc1l1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Vkorc1l1 (69568)
Length:
4935
CDS:
64..594

Additional Resources:

NCBI RefSeq record:
NM_027121.4
NBCI Gene record:
Vkorc1l1 (69568)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_027121.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042239 CTCCTCATCATCAATTACAAA pLKO.1 517 CDS 100% 5.625 7.875 N Vkorc1l1 n/a
2 TRCN0000046349 TGGTGTATTAAACCAGCCAAA pLKO.1 303 CDS 100% 4.050 5.670 N VKORC1L1 n/a
3 TRCN0000042240 CGTCACCACATATGTGCTGAA pLKO.1 489 CDS 100% 0.000 0.000 N Vkorc1l1 n/a
4 TRCN0000046348 CCTGGCCTACATTCTGTACTT pLKO.1 441 CDS 100% 4.950 3.960 N VKORC1L1 n/a
5 TRCN0000046350 ACAGTGTCTTTGGACTTATAT pLKO.1 323 CDS 100% 15.000 10.500 N VKORC1L1 n/a
6 TRCN0000433447 CAATCAAAGACAAGCTTTAAC pLKO_005 815 3UTR 100% 13.200 9.240 N VKORC1L1 n/a
7 TRCN0000042238 CCTCAGAATCTTAAACTGATT pLKO.1 704 3UTR 100% 4.950 3.465 N Vkorc1l1 n/a
8 TRCN0000042241 CATTCTGTACTTTGTGCTGAA pLKO.1 450 CDS 100% 4.050 2.835 N Vkorc1l1 n/a
9 TRCN0000046352 GCTCTCCATCTACGCCTACCA pLKO.1 147 CDS 100% 0.880 0.616 N VKORC1L1 n/a
10 TRCN0000042242 CCTGCTCTCCATCTACGCCTA pLKO.1 144 CDS 100% 0.720 0.504 N Vkorc1l1 n/a
11 TRCN0000046351 CCAAACAGTGTCTTTGGACTT pLKO.1 319 CDS 100% 0.405 0.284 N VKORC1L1 n/a
12 TRCN0000414870 ACAGTTATTACTTGGCATGAC pLKO_005 354 CDS 100% 4.050 2.430 N VKORC1L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_027121.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05083 pDONR223 100% 92% 97.1% None (many diffs) n/a
2 ccsbBroad304_05083 pLX_304 0% 92% 97.1% V5 (many diffs) n/a
3 TRCN0000468462 ACAGGGTCTATCTTCCCTAACTAG pLX_317 66.6% 92% 97.1% V5 (many diffs) n/a
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