Construct: ORF TRCN0000468464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018380.1_s317c1
- Derived from:
- ccsbBroadEn_06990
- DNA Barcode:
- CACACTGGGAAGAATCTGCAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPOCK1 (6695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6695 | SPOCK1 | SPARC (osteonectin), cwcv a... | NM_004598.4 | 99.8% | 99.5% | 229C>A;442T>C |
| 2 | mouse | 20745 | Spock1 | sparc/osteonectin, cwcv and... | NM_001166463.1 | 89.5% | 94.7% | (many diffs) |
| 3 | mouse | 20745 | Spock1 | sparc/osteonectin, cwcv and... | NM_009262.3 | 88.9% | 94.1% | (many diffs) |
| 4 | mouse | 20745 | Spock1 | sparc/osteonectin, cwcv and... | NM_001166466.1 | 88.7% | 93.8% | (many diffs) |
| 5 | mouse | 20745 | Spock1 | sparc/osteonectin, cwcv and... | NM_001166465.1 | 72.5% | 67.4% | (many diffs) |
| 6 | mouse | 20745 | Spock1 | sparc/osteonectin, cwcv and... | NM_001166464.1 | 72.1% | 67% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1383
- ORF length:
- 1317
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggcgatcgcg gtgttggcgg cggccgccgc ggcgtggtgc ttcctccaag 121 tcgagagccg gcacctggac gcgctcgccg gaggcgcggg ccccaaccac ggcaatttcc 181 tagacaatga ccagtggctg agcaccgtct cccagtacga ccgggacaag tactggaacc 241 gctttcgaga cgatgattat ttcagaaact ggaatcccaa caagcccttt gacaaagccc 301 tggacccatc caaggacccc tgcctgaagg taaaatgcag ccctcacaaa gtgtgtgtga 361 cccaggacta ccagaccgcc ctgtgtgtca gccgcaagca cctgctcccc aggcaaaaga 421 aggggaacgt ggcccagaaa cactgggttg gaccttcgaa tttggtcaag tgcaagccct 481 gtcccgtggc acagtcagcc atggtccgcg gctcagatgg ccactcctac acatccaagt 541 gcaaattgga gttccatgct tgttctactg gcaaaagcct cgccaccctc tgtgatgggc 601 cctgtccctg tctcccagag cctgagccac caaagcacaa ggcagaaagg agtgcctgca 661 cagacaagga gttgcggaac cttgcctccc ggctgaagga ttggtttgga gctctccacg 721 aggatgcgaa cagagtcatc aagcccacca gctccaacac agcccaaggc aggtttgaca 781 ctagcatcct gcccatctgc aaggactccc tgggctggat gttcaacaag ttggacatga 841 actatgacct cctgcttgac ccttcagaga tcaatgccat ctacctggat aagtacgagc 901 cctgtatcaa gcctcttttc aactcgtgtg actccttcaa ggatggcaag ctttctaaca 961 atgagtggtg ctactgcttc cagaagcctg gaggtctccc ttgccagaat gaaatgaaca 1021 gaattCAGAA GCTGAGTAAG GGGAAAAGCC TGTTGGGGGC CTTCATACCT CGGTGTAATG 1081 AGGAGGGCTA TTACAAAGCC ACACAGTGCC ACGGCAGCAC GGGGCAGTGC TGGTGTGTGG 1141 ACAAATATGG GAATGAGTTG GCTGGCTCCA GGAAACAGGG TGCTGTGAGC TGTGAAGAGG 1201 AGCAGGAAAC CTCAGGGGAT TTTGGCAGTG GTGGGTCCGT GGTCCTGCTG GATGACCTAG 1261 AATATGAACG GGAGCTGGGA CCAAAGGACA AAGAGGGGAA GCTGAGGGTG CACACCCGAG 1321 CCGTGACAGA GGATGATGAG GATGAGGATG ATGACAAAGA GGATGAGGTC GGGTACATAT 1381 GGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1441 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1501 GGCTTTATAT ATCTTGTGGA AAGGACGACA CACTGGGAAG AATCTGCAAT TAACGCGTTA 1561 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt