Construct: ORF TRCN0000468480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016881.1_s317c1
- Derived from:
- ccsbBroadEn_00544
- DNA Barcode:
- AATACAGTTAAAGCCCATGCAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCER1A (2205)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2205 | FCER1A | Fc fragment of IgE receptor Ia | NM_002001.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcctgccatg gaatccccta ctctactgtg tgtagcctta ctgttcttcg 121 ctccagatgg cgtgttagca gtccctcaga aacctaaggt ctccttgaac cctccatgga 181 atagaatatt taaaggagag aatgtgactc ttacatgtaa tgggaacaat ttctttgaag 241 tcagttccac caaatggttc cacaatggca gcctttcaga agagacaaat tcaagtttga 301 atattgtgaa tgccaaattt gaagacagtg gagaatacaa atgtcagcac caacaagtta 361 atgagagtga acctgtgtac ctggaagtct tcagtgactg gctgctcctt caggcctctg 421 ctgaggtggt gatggagggc cagcccctct tcctCAGGTG CCATGGTTGG AGGAACTGGG 481 ATGTGTACAA GGTGATCTAT TATAAGGATG GTGAAGCTCT CAAGTACTGG TATGAGAACC 541 ACAACATCTC CATTACAAAT GCCACAGTTG AAGACAGTGG AACCTACTAC TGTACGGGCA 601 AAGTGTGGCA GCTGGACTAT GAGTCTGAGC CCCTCAACAT TACTGTAATA AAAGCTCCGC 661 GTGAGAAGTA CTGGCTACAA TTTTTTATCC CATTGTTGGT GGTGATTCTG TTTGCTGTGG 721 ACACAGGATT ATTTATCTCA ACTCAGCAGC AGGTCACATT TCTCTTGAAG ATTAAGAGAA 781 CCAGGAAAGG CTTCAGACTT CTGAACCCAC ATCCTAAGCC AAACCCCAAA AACAACTGCC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GAAATACAGT TAAAGCCCAT GCAATTACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt