Construct: ORF TRCN0000468501
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003236.1_s317c1
- Derived from:
- ccsbBroadEn_00284
- DNA Barcode:
- CGTCATAAGTGGAAGGGTTTCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKN2D (1032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468501
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1032 | CDKN2D | cyclin dependent kinase inh... | NM_001800.4 | 99.7% | 100% | 90G>C |
| 2 | human | 1032 | CDKN2D | cyclin dependent kinase inh... | NM_079421.3 | 99.7% | 100% | 90G>C |
| 3 | mouse | 12581 | Cdkn2d | cyclin-dependent kinase inh... | NM_009878.3 | 82.1% | 86.7% | (many diffs) |
| 4 | mouse | 12581 | Cdkn2d | cyclin-dependent kinase inh... | XM_006509968.1 | 82.1% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 564
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctggaggag gttcgcgccg gcgaccggct gagtggggcg gcggcccggg 121 gcgacgtgca ggaggtgcgc cgccTTCTGC ACCGCGAGCT GGTGCATCCC GACGCCCTCA 181 ACCGCTTCGG CAAGACGGCG CTGCAGGTCA TGATGTTTGG CAGCACCGCC ATCGCCCTGG 241 AGCTGCTGAA GCAAGGTGCC AGCCCCAATG TCCAGGACAC CTCCGGTACC AGTCCAGTCC 301 ATGACGCAGC CCGCACTGGA TTCCTGGACA CCCTGAAGGT CCTAGTGGAG CACGGGGCTG 361 ATGTCAACGT GCCTGATGGC ACCGGGGCAC TTCCAATCCA TCTGGCAGTT CAAGAGGGTC 421 ACACTGCTGT GGTCAGCTTT CTGGCAGCTG AATCTGATCT CCATCGCAGG GACGCCAGGG 481 GTCTCACACC CTTGGAGCTG GCACTGCAGA GAGGGGCTCA GGACCTCGTG GACATCCTGC 541 AGGGCCACAT GGTGGCCCCG CTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GTCATAAGTG 721 GAAGGGTTTC TGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att