Construct: ORF TRCN0000468526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003227.1_s317c1
- Derived from:
- ccsbBroadEn_07084
- DNA Barcode:
- GAAATATTTGGATCGAAACTACGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLEC3B (7123)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7123 | CLEC3B | C-type lectin domain family... | NM_003278.3 | 99.6% | 99% | 10T>G;316G>A |
| 2 | human | 7123 | CLEC3B | C-type lectin domain family... | XM_017007116.1 | 83.3% | 82.6% | 10T>G;108_109ins99;217G>A |
| 3 | human | 7123 | CLEC3B | C-type lectin domain family... | NM_001308394.2 | 79% | 78.7% | 0_1ins126;190G>A |
| 4 | human | 7123 | CLEC3B | C-type lectin domain family... | XM_017007117.2 | 74.2% | 66.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 672
- ORF length:
- 606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctcgggggg gcctacctcc tcctctgcct cttctccctc ctgacccagg 121 tcaccaccga gccaccaacc cagaagccca agaagattgt aaatgccaag aaagatgttg 181 tgaacacaaa gatgtttgag gagctcaaga gccgtctgga caccctggcc caggaggtgg 241 cccTGCTGAA GGAGCAGCAG GCCCTGCAGA CGGTCTGCCT GAAGGGGACC AAGGTGCACA 301 TGAAATGCTT TCTGGCCTTC ACCCAGACGA AGACCTTCCA CGAGGCCAGC GAGGACTGCA 361 TCTCGCGCGG GGGCACCCTG AGCACCCCTC AGACTGGCTC GGAGAACGAC GCCCTGTATG 421 AGTACCTGCG CCAGAGCGTG GGCAACGAGG CCGAGATCTG GCTGGGCCTC AACGACATGG 481 CGGCCGAGGG CACCTGGGTG GACATGACCG GCGCCCGCAT CGCCTACAAG AACTGGGAGA 541 CTGAGATCAC CGCGCAACCC GATGGCGGCA AGACCGAGAA CTGCGCGGTC CTGTCAGGCG 601 CGGCCAACGG CAAGTGGTTC GACAAGCGCT GCCGCGATCA GCTGCCCTAC ATCTGCCAGT 661 TCGGGATCGT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAA ATATTTGGAT CGAAACTACG 841 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t