Construct: ORF TRCN0000468527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000842.1_s317c1
- Derived from:
- ccsbBroadEn_08144
- DNA Barcode:
- TACGCTCCTTCCTAAAGCTCCAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERTAD3 (29946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29946 | SERTAD3 | SERTA domain containing 3 | NM_013368.3 | 99.8% | 99.4% | 7G>C |
| 2 | human | 29946 | SERTAD3 | SERTA domain containing 3 | NM_203344.3 | 99.8% | 99.4% | 7G>C |
| 3 | human | 29946 | SERTAD3 | SERTA domain containing 3 | XM_005258832.4 | 99.8% | 99.4% | 7G>C |
| 4 | human | 29946 | SERTAD3 | SERTA domain containing 3 | XM_006723179.3 | 99.8% | 99.4% | 7G>C |
| 5 | mouse | 170742 | Sertad3 | SERTA domain containing 3 | NM_133210.2 | 83.4% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 654
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcgaggcttg aagaggaaac actctgattt ggaagaggag gaggagaggt 121 gggagtggag tccagcaggc cttcagagct accagcaagc cctgctccgc atctccctag 181 acaaagtcca gcgcagcctg ggcccccgag cacccagccT CCGCAGGCAT GTCCTCATCC 241 ATAACACCCT CCAACAGCTG CAGGCTGCAC TTCGCCTGGC TCCCGCCCCT GCCCTGCCCC 301 CCGAGCCCCT CTTCCTGGGC GAGGAGGATT TCTCCCTGTC AGCCACCATT GGCTCTATCC 361 TCAGGGAGCT GGACACCTCC ATGGATGGGA CTGAGCCCCC TCAGAATCCA GTGACTCCCC 421 TTGGCCTCCA GAATGAAGTG CCACCCCAGC CTGATCCAGT CTTCTTAGAA GCTCTGAGCT 481 CCCGGTACTT GGGGGACTCT GGCCTGGATG ACTTCTTTCT GGACATTGAC ACATCTGCGG 541 TAGAAAAGGA GCCTGCACGG GCCCCACCAG AGCCTCCTCA CAACCTCTTC TGTGCCCCAG 601 GTTCTTGGGA GTGGAATGAA CTGGATCACA TCATGGAAAT CATTCTGGGG TCCTACCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAT ACGCTCCTTC CTAAAGCTCC AGTACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att