Construct: ORF TRCN0000468554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012813.1_s317c1
- Derived from:
- ccsbBroadEn_09807
- DNA Barcode:
- CGTAGTACTGGTTAACAACTGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM17 (200728)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200728 | TMEM17 | transmembrane protein 17 | NM_198276.3 | 99.3% | 98.9% | (many diffs) |
| 2 | human | 200728 | TMEM17 | transmembrane protein 17 | XM_024452750.1 | 86% | 83.3% | (many diffs) |
| 3 | human | 200728 | TMEM17 | transmembrane protein 17 | XM_011532693.2 | 65% | 58.1% | (many diffs) |
| 4 | human | 200728 | TMEM17 | transmembrane protein 17 | XM_011532694.2 | 58.9% | 49.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgccagat ccggtgcgcc agcggctggg naacttcagc cgggccgtgt 121 tcagtgattc caatcggacc agtccagagt ccaatgaggg tccggaaaat gaaatggtct 181 ccagtttGGC ACTGCAGATG TCACTTTATT TTAATACCTA CTATTTCCCA CTGTGGTGGG 241 TGAGCAGCAT TATGATGCTT CACATGAAGT ATTCAATCTT GCCTGACTAC TACAAATTCA 301 TTGTGATCAC TGTTATCATC CTAATAACCT TAATTGAAGC CATCCGGTTG TATCTGGGCT 361 ACGTGGGTAA CCTACAGGAG AAGGTTCCTG AGTTGGCTGG CTTTTGGCTT TTGAGCCTTC 421 TATTGCAGTT ACCTTTAATT CTTTTCTTGC TCTTTAATGA AGGCCTAACA AATCTGCCCT 481 TGGAAAAAGC GATACATATC ATCTTCACTC TCTTCCTTGC TTTCCAAGTT GTTGCAGCAT 541 TTCTTACCTT AAGGAAAATG GTTAATCAGT TGGCAGTTCG TTTCCACCTC CAAGACTTTG 601 ACCGGCTCTC TGCAAACAGA GGAGACATGA GAAGGATGAG GTCATGTATA GAAGAGATCT 661 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGACGTAG TACTGGTTAA CAACTGCGGA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt