Construct: ORF TRCN0000468587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014080.1_s317c1
- Derived from:
- ccsbBroadEn_16165
- DNA Barcode:
- AGTAGAGTCACTATGTGCAGACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH2NLA (388677)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364012.2 | 99.7% | 99.5% | 285A>G;473C>T |
| 2 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_203458.4 | 99.7% | 99.5% | 285A>G;473C>T |
| 3 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364008.1 | 94.5% | 94.7% | 1_39del;228A>G;324A>G |
| 4 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_001364006.1 | 94.5% | 94.3% | 1_39del;324A>G;512C>T |
| 5 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364007.2 | 85.5% | 85.8% | 1_117del;306A>G;402A>G |
| 6 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364013.1 | 80.3% | 80.2% | 1_171del;456A>G;644C>T |
| 7 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_006711700.4 | 78.1% | 78.4% | 1_195del;384A>G;480A>G |
| 8 | human | 101929796 | NOTCH2NLR | notch 2 N-terminal like R | NR_157049.1 | 61.6% | (many diffs) | |
| 9 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_011510256.2 | 57.1% | 57.2% | 1_528del;717A>G;813A>G |
| 10 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452030.1 | 51.5% | 51.8% | (many diffs) |
| 11 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452031.1 | 51.5% | 51.8% | (many diffs) |
| 12 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452032.1 | 51.5% | 51.8% | (many diffs) |
| 13 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452035.1 | 51.5% | 51.8% | (many diffs) |
| 14 | human | 4853 | NOTCH2 | notch receptor 2 | NM_001200001.1 | 18.2% | 17.2% | (many diffs) |
| 15 | human | 4853 | NOTCH2 | notch receptor 2 | NM_024408.4 | 9.1% | 8.6% | (many diffs) |
| 16 | mouse | 18129 | Notch2 | notch 2 | NM_010928.2 | 8% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgttacctac cacaatggca caggatactg caaatgtcca gaaggcttct 121 tgggggaata ttgtcaacat cgagacccct gtgagaagaa ccgctgccag aatggtggga 181 cttgtgtggc ccaggccatg ctggggaaag ccacgtgccg atgtgcctca gggtttacag 241 gagaggactg ccagtactcg acatctcatc catgctttgt gtctcgacct tgcctgaatg 301 gcggcacatg ccatatgctc agccgggata cctatgagtg cacctgtcag gtcgggttta 361 caggtaagga gtgccaatgg accgatgcct gcctgtctca tccctgtgca aatggaagta 421 cctgtaccac tgtggccaac cagttcTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA 481 AGTGTGAGAC TGATGTCAAT GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCATCT 541 GCCTCAACCT GCCTGGTTCC TACCAGTGCC AGTGCCTTCA GGGCTTCACA GGCCAGTACT 601 GTGACAGCCT GTATGTGCCC TGTGCACCCT CGCCTTGTGT CAATGGAGGC ACCTGTCGGC 661 AGACTGGTGA CTTCACTTTT GAGTGCAACT GCCTTCCAGA AACAGTGAGA AGAGGAACAG 721 AGCTCTGGGA AAGAGACAGG GAAGTCTGGA ATGGAAAAGA ACACGATGAG AATTACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAA GTAGAGTCAC TATGTGCAGA CGGACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att