Construct: ORF TRCN0000468593
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000648.1_s317c1
- Derived from:
- ccsbBroadEn_05313
- DNA Barcode:
- TTTCTTGGCTTGCACTGTGACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT14 (256281)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468593
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256281 | NUDT14 | nudix hydrolase 14 | NM_177533.5 | 100% | 100% | |
| 2 | human | 256281 | NUDT14 | nudix hydrolase 14 | NM_001318380.1 | 65.6% | 65.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 732
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgcatcgag ggggcgtccg tgggccgctg cgccgcctca ccctacctgc 121 ggccgctcac gctgcattac cgccagaatg gtgcccagaa gtcctgggac ttcatgaaga 181 cgcatgacag cgtgaccgtt ctcttattca actcttctcg gaggagcctg gtgttggtga 241 agcagttccg gccagctgtg tatgcgggtg aggtggagcg ccgcttccca gggtccctag 301 cagctgtaga ccaggacggg cctcgggagc tacagccagc cctgcccggc TCAGCGGGGG 361 TGACAGTTGA GCTGTGTGCC GGCCTCGTGG ACCAGCCTGG GCTCTCGCTG GAGGAAGTGG 421 CTTGCAAGGA GGCTTGGGAG GAGTGTGGCT ACCACTTGGC CCCCTCTGAT CTGCGCCGGG 481 TCGCCACATA CTGGTCTGGA GTGGGACTGA CTGGCTCCAG ACAGACCATG TTCTACACAG 541 AGGTGACAGA TGCCCAGCGT AGCGGTCCAG GTGGGGGCCT GGTGGAGGAG GGTGAGCTCA 601 TTGAGGTGGT GCACCTGCCC CTGGAAGGCG CCCAGGCCTT TGCAGACGAC CCGGACATCC 661 CCAAGACCCT CGGCGTCATC TTTGGTGTCT CATGGTTCCT CAGCCAGGTG GCCCCCAACC 721 TGGATCTCCA GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT CTTGGCTTGC ACTGTGACCG 901 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t