Construct: ORF TRCN0000468621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014867.1_s317c1
- Derived from:
- ccsbBroadEn_11672
- DNA Barcode:
- TGTAAACACTATGACCAATGATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM35 (23087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23087 | TRIM35 | tripartite motif containing 35 | NM_001304495.2 | 67.9% | 60.1% | (many diffs) |
| 2 | human | 23087 | TRIM35 | tripartite motif containing 35 | NM_001362813.2 | 50.2% | 51.8% | 434_435ins112;507_894del |
| 3 | human | 23087 | TRIM35 | tripartite motif containing 35 | NM_171982.5 | 31.8% | 31.5% | 434_435ins112;507_1479del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcggagtccc gacgtgtccc ccgggccttc ccgctccttc aaggaggagt 121 tgctctgcgc cgtctgctac gaccccttcc gcgacgcagt cactctgcgc tgcggccaca 181 acttctgccg cgggtgcgtg agccgctgct gggaggtgca ggtgtcgccc acctgcccag 241 tgtgcaaaga ccgcgcgtca cccgccgacc tgcgcaccaa ccacaccctc aacaacctgg 301 tggagaagct gctgcgcgag gaggccgagg gcgcgcgctg gaccagctac cgcttctcgc 361 gtgtctgccg ccTGCACCGC GGACAGCTCA GCCTCTTCTG CCTCGAGGAC AAGGAGCTGC 421 TGTGCTGCTC CTGCCAGGCC GACCCCCGAC ACCAGGGGCA CCGCGTGCAG CCGGTGAAGG 481 ACACTGCCCA CGACTTTCGG GTGAGGAGCC TGATAGCCGA AGAGAGAAGG AACTTTTTAC 541 CAACTCACCA GTGGATAGTG ACAAAGACAA GGCTCCAAAC CAGCTCTCCT AACCTTCAGT 601 CCAGGAGGCA AGGCCAAGTG CAGGAACATG GAGCATGCAC TGCGGGAGAA GGCCAAGGCC 661 TTCTGGGCCA TGCGGCGCTC CTATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTAAACACTA 841 TGACCAATGA TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att