Transcript: Human NM_171982.5

Homo sapiens tripartite motif containing 35 (TRIM35), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
TRIM35 (23087)
Length:
4183
CDS:
38..1519

Additional Resources:

NCBI RefSeq record:
NM_171982.5
NBCI Gene record:
TRIM35 (23087)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_171982.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033780 CTTGCATCTGTGGAATCTGTA pLKO.1 923 CDS 100% 4.950 3.465 N TRIM35 n/a
2 TRCN0000033779 CATGAAACACAAGAGCCGAAA pLKO.1 796 CDS 100% 4.050 2.835 N TRIM35 n/a
3 TRCN0000033782 CGCGTCTGGAAGAAGATGCTT pLKO.1 905 CDS 100% 3.000 2.100 N TRIM35 n/a
4 TRCN0000033781 AGTGTCAAGGAAGAACTGGAT pLKO.1 1493 CDS 100% 2.640 1.848 N TRIM35 n/a
5 TRCN0000033783 GCGCTGGACCAGCTACCGCTT pLKO.1 307 CDS 100% 0.000 0.000 N TRIM35 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_171982.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11672 pDONR223 100% 31.8% 31.5% None 434_435ins112;507_1479del n/a
2 ccsbBroad304_11672 pLX_304 0% 31.8% 31.5% V5 434_435ins112;507_1479del n/a
3 TRCN0000468621 TGTAAACACTATGACCAATGATTG pLX_317 50.4% 31.8% 31.5% V5 434_435ins112;507_1479del n/a
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