Construct: ORF TRCN0000468631
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001599.1_s317c1
- Derived from:
- ccsbBroadEn_13411
- DNA Barcode:
- ATCTCTGTCTTTGTGCACACTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBY2 (220082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468631
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220082 | CBY2 | chibby family member 2 | NM_001286342.1 | 100% | 100% | |
| 2 | human | 220082 | CBY2 | chibby family member 2 | NM_001286341.2 | 96.1% | 94.3% | (many diffs) |
| 3 | human | 220082 | CBY2 | chibby family member 2 | NM_152719.3 | 90% | 88.8% | (many diffs) |
| 4 | human | 220082 | CBY2 | chibby family member 2 | XM_011534971.2 | 86.7% | 86.7% | 1_189del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1302
- ORF length:
- 1236
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca accagagggg ttgaaatgta ggatggagga atccaatgca gagaggggca 121 cagccgaacc cttcccgagg ctccacaact tgtacagcac ccctcgctgc gcgcagcagg 181 ccgccctgcc ccggctgagc cgcaggatgg cgagccagca ctcctatcca ctgaaccgct 241 tctcctccgt gcctttagac cccatggagc gccccatgtc ccaggccgac ctggagctgg 301 actacaaccc gccgcgggtg cagctcagcg acgagatgtt cgtgttccag gacgggcgct 361 gggtaaatga gaactgccgc ctgcagtctc cctacttctc cccatccgcc tccttccacc 421 acaagctgca ccacaagagg ctggccaagg agtgcatgct gcaggaggag aacaagtctc 481 tgcgggagga gaacaaggcc ctgcgcgagg agaaccggat gctcagcaag gagaacaaga 541 tcctacaggt cttctgggag gagcacaagg cctcgctggg ccgagaggag agccgggccc 601 cctcgccact gctgcacaaa gacagcgcgt ccctggaggt ggtgaagaag gaccacgtcg 661 ccctgcaggt gccccgtggc aaggaggaca gcaccctgca gctcctccgg gaggagaatc 721 gcgcgctgca gcagctgctg gagcagaaac aggcctactg ggcgcaggca gaggacacgg 781 ccgcccctgc cgaggaaagc aagcccgccc cctcacccca cgaggagccc tgcagccccg 841 ggctgctgca ggaccagggc tccggcctct cctcccgctt cgaggagccc aaagggcctc 901 cggcccggca ggaggactcc aaggagctgc gcgccctgcg gaagatggtc agcaacatgt 961 ccgggcccTC CGGGGAGGAG GAGGCCAAGG TGGGCCCGGG CCTGCCCGAC GGCTGCCAGC 1021 CCCTGCAGCT GCTGAGAGAG ATGAGGCAGG CGCTGCAGGC CCTGCTCAAG GAGAACCGGC 1081 TCCTGCAGGA GGAGAACAGG ACCCTGCAGG TGCTACGGGC AGAGCACAGG GGCTTCCAGG 1141 AGGAGAACAA GGCCCTGTGG GAGAACAACA AGCTGAAGCT GCAGCAGAAG CTGGTCATTG 1201 ACACCGTGAC CGAGGTCACC GCGCGCATGG AAATGCTCAT CGAGGAGCTC TACGCCTTCA 1261 TGCCGGCCAG GAGCCAGGAC CCCAAGAAGC CTAGCAGGGT CTGCCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGAATC TCTGTCTTTG TGCACACTGG GACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t