Construct: ORF TRCN0000468636
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014680.1_s317c1
- Derived from:
- ccsbBroadEn_02254
- DNA Barcode:
- ACACCGCTACCATTGGGTAACTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGP1 (9827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468636
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9827 | RGP1 | RGP1 homolog, RAB6A GEF com... | NM_001080496.3 | 100% | 100% | |
| 2 | mouse | 242406 | Rgp1 | RAB6A GEF compex partner 1 | NM_172866.3 | 88.8% | 96.9% | (many diffs) |
| 3 | mouse | 242406 | Rgp1 | RAB6A GEF compex partner 1 | XM_006537909.3 | 88.8% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1239
- ORF length:
- 1173
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tgaagtggta gcagagctca gccggggtcc tgtatttttg gctggggagg 121 cgctggagtg tgtagtgacc gtcaccaacc cccttccgcc cacggccact tctgcatcca 181 gtgaggccct ggcctgggcc agtgcccaaa tccactgcca gttccatgcc agtgagagtc 241 gagtagcact gcctcctcct gactctagtc agccagatgt ccagcccgac agccagactg 301 tctttctgcc acaccgaggt gagaggggcc agtgtatcct ttctactcca ccgaaaattc 361 tattctgtga cctgaggctt gatcctggag agtccaaatc atactcctac agtgaagtgc 421 tgcccataga gggaccaccc tcctttcggg gtcagtcagt caagtacgtc tacaaactga 481 ccattggctg ccagcgtgtc aactccccta tcactttact cagagtccct ctgagggttc 541 ttgtgctgac tggccttcag gatgtccggt ttccccagga tgaggctgta gccccatcca 601 gtccattctt ggaggaggat gaaggtggga agaaagattc atggctagct gagctggctg 661 gggaacgcct aatggctgcc acatcctgcc gcagcctcca tctatacaat atcagtgatg 721 gccgagggaa agttgggacg tttggcatct tcaaatctgt gtacagactt ggcgaggacg 781 tggtggggac cttaaactta gggGAAGGAA CCGTAGCTTG TTTGCAGTTT TCAGTCAGCT 841 TACAGACCGA GGAGCGTGTA CAGCCTGAGT ACCAGCGGCG ACGTGGGGCA GGGGGTGTCC 901 CCTCTGTGTC ACATGTGACT CACGCCCGGC ACCAGGAATC CTGCCTACAT ACAACTAGAA 961 CCAGCTTCTC CCTCCCAATC CCTCTCAGCT CCACCCCAGG CTTCTGTACA GCCATTGTGT 1021 CCTTGAAGTG GAGATTGCAT TTTGAATTTG TAACGTCCCG AGAACCAGGA TTGGTACTCC 1081 TACCCCCTGT GGAACAGCCC GAACCTACCA CCTGGACAGG ACCTGAGCAA GTACCTGTAG 1141 ACACCTTCAG CTGGGACCTG CCCATCAAGG TGCTGCCTAC TAGCCCCACC CTGGCCTCAT 1201 ATGCTGCCCC AGGCCCCAGC ACCAGCACCA TAACCATCTG CCCAACTTTC TTGTACAAAG 1261 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1321 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1381 ACGAACACCG CTACCATTGG GTAACTCCAC GCGTTAAGTC gacaatcaac ctctggatta 1441 caaaatttgt gaaagatt