Construct: ORF TRCN0000468637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010283.1_s317c1
- Derived from:
- ccsbBroadEn_01727
- DNA Barcode:
- CAATGCCATCCCTGTATGGGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUFT1 (7286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468637
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7286 | TUFT1 | tuftelin 1 | NM_020127.3 | 100% | 100% | |
| 2 | human | 7286 | TUFT1 | tuftelin 1 | XM_017002223.2 | 94.9% | 94.8% | 1_2delATins59 |
| 3 | human | 7286 | TUFT1 | tuftelin 1 | NM_001126337.2 | 93.5% | 93.5% | 59_60ins75 |
| 4 | human | 7286 | TUFT1 | tuftelin 1 | NM_001301317.2 | 93.3% | 90.7% | (many diffs) |
| 5 | human | 7286 | TUFT1 | tuftelin 1 | XM_017002224.1 | 88.6% | 88.4% | 0_1ins132;2T>A |
| 6 | human | 7286 | TUFT1 | tuftelin 1 | XM_011509961.2 | 84.8% | 84.8% | 59_60ins177 |
| 7 | mouse | 22156 | Tuft1 | tuftelin 1 | NM_011656.3 | 89.3% | 88.9% | (many diffs) |
| 8 | mouse | 22156 | Tuft1 | tuftelin 1 | NM_001293728.1 | 83.1% | 82.8% | (many diffs) |
| 9 | mouse | 22156 | Tuft1 | tuftelin 1 | XM_006501307.1 | 76.7% | 76.4% | (many diffs) |
| 10 | mouse | 22156 | Tuft1 | tuftelin 1 | XM_006501308.2 | 76.7% | 76.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgggacgcgg aactggtgta ccctggtgga cgtgcaccca gaggaccagg 121 cggcgggcag cgtggacatt ctcaggctga ctctccaggg tgaactgaca ggagatgaac 181 ttgaacacat agcccagaag gcgggcagga agacctatgc catggtgtcc agccactcag 241 ctggtcattc tctggcttca gaactggtgg agtcccatga tggacatgag gagatcatta 301 aggtgtactt gaaggggagg tctggagaca agatgattca cgagaagaat attaaccagc 361 tgaagagtga ggtccagtac atccaggagg ccaggaactg cctacagaag ctccgggagg 421 atataagtag caagcttgac aggaacctag gagattctct ccatcgacag gagatacagg 481 tggtgctaga aaagccaaat ggctttagtc agagtcccac agccctgtac agcagcccac 541 ctgaggtgga cacctgtata aatgaggatg ttgagagctt gaggaagacg gtgcaggact 601 tgctggccaa gcttcaggag gccaagcggc aacaccagtc agactgtgtg gcttttgagg 661 tcacactcag ccggtaccag agggaagcag aacaaagtaa tgtggccctt cagagagagg 721 aggacagagt ggagcagaaa gaggcagaag tcggagagct gcagaggcgc ttgctaggga 781 tggagacgga gcatcaggCC TTACTGGCGA AAGTGAGGGA AGGGGAGGTG GCCCTAGAGG 841 AACTTCGGAG CAACAATGCT GACTGCCAAG CAGAACGAGA AAAGGCTGCT ACCCTGGAAA 901 AGGAAGTGGC CGGGTTGCGG GAGAAGATCC ACCACTTGGA TGACATGCTC AAGAGCCAGC 961 AGCGGAAAGT CCGGCAAATG ATAGAGCAGC TCCAGAATTC AAAAGCTGTG ATCCAGTCAA 1021 AGGACGCCAC CATCCAGGAG CTCAAGGAGA AAATCGCCTA TCTGGAGGCA GAGAATTTAG 1081 AGATGCATGA CCGGATGGAA CACCTGATAG AAAAACAAAT CAGTCATGGC AACTTCAGCA 1141 CCCAGGCCCG GGCCAAGACA GAGAACCCGG GCAGTATTAG GATATCCAAG CCGCCTAGCC 1201 CGAAGCCCAT GCCTGTCATC CGAGTGGTGG AAACCTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 ACAATGCCAT CCCTGTATGG GATCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt