Construct: ORF TRCN0000468645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005458.1_s317c1
- Derived from:
- ccsbBroadEn_09863
- DNA Barcode:
- ATTAGCCTTACCTCTTTATTACTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C10orf25 (220979)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220979 | C10orf25 | chromosome 10 open reading ... | NR_126421.2 | 36.6% | 0_1ins56;132T>A;311_786del | |
| 2 | human | 220979 | C10orf25 | chromosome 10 open reading ... | NR_164113.1 | 10.1% | 0_1ins56;132T>A;311_2993del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 435
- ORF length:
- 366
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcccgggc ccacccgaaA GCGTTGTTCG TTTCTTCCTG TGGTTTTGTT 121 TTCTCCTCCC CCCAACTCGG AAGGCGTCCT GTGACCCCCG GGACCTCAAG AGCTGTAACC 181 GCCCCTGTGT GTGGTCGAGG CTTTTGAAAC CAAACTCTAG CCTCTCCAAC CTAGAAACTG 241 CATACTTTCC ACAAAATTTG AGATTTTTGA GACCCTGGTA CTTTTCACGA AGTCACCTTA 301 ATTATCACCA AAAAGCTCCT GCAAGATGGG AGTGGTTATA CTCCATTTAC AGGAAAGGAA 361 CCAAGGCTCA GAGAAGAAAT GTGCTCCGTT CACCGTGTGC TCCTCCTCAG CCATCCTGGC 421 CGTGCTCAGT CATCTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 481 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 541 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTAGCCTTA CCTCTTTATT 601 ACTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt