Construct: ORF TRCN0000468668
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010624.1_s317c1
- Derived from:
- ccsbBroadEn_03211
- DNA Barcode:
- AGACCAGCGTATAACAAGCTCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LACTB2 (51110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468668
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51110 | LACTB2 | lactamase beta 2 | NM_016027.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgtactg cagcgcgtcg agcggctgtc caatcgagtc gtgcgtgtgt 121 tgggctgtaa cccgggtccc atgaccctcc aaggcaccaa cacctaccta gtggggaccg 181 gccccaggag aatcctcatt gacactggag aaccagcaat tccagaatac atcagctgtt 241 taaagcaggc tctaactgaa tttaacacag caatccagga aattgtagtg actcactggc 301 accgagatca ttctggaggc ataggagata tttgtaaaag catcaataat gacactacct 361 attgcattaa aaaactccca cggaatcctc agagagaaga aattatagga aatggagagc 421 aacaatatgt ttatctgaaa gatggagatg tgattaagac tgagggagcc actctaagag 481 ttctatatac ccctggccac actgatgatc acatggctct actcttagaa gaggaaaatg 541 ctatcttttc tggagattgc atcctagggg aaggaacaac ggtatttgaa gacctctatg 601 attatatgaa ctctttaaaa gagttattga aaatcaaagc tgatattata tatccaggac 661 atggcccagt aattcataat gctgaagcta aaattcaaca atacatttct CACAGAAATA 721 TTCGAGAGCA GCAAATTCTT ACATTATTTC GTGAGAACTT TGAGAAATCA TTTACAGTAA 781 TGGAGCTTGT AAAAATTATT TACAAGAATA CTCCTGAGAA TTTACATGAA ATGGCTAAAC 841 ATAATCTCTT ACTTCATTTG AAAAAACTAG AAAAAGAAGG AAAAATATTT AGCAACACAG 901 ATCCTGACAA GAAATGGAAA GCTCATCTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGCC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGACC 1081 AGCGTATAAC AAGCTCTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt