Construct: ORF TRCN0000468671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015614.1_s317c1
- Derived from:
- ccsbBroadEn_03641
- DNA Barcode:
- TTGACAGTTCTTTACTCCCTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASF1B (55723)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55723 | ASF1B | anti-silencing function 1B ... | NM_018154.3 | 100% | 100% | |
| 2 | mouse | 66929 | Asf1b | anti-silencing function 1B ... | NM_024184.2 | 87.8% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 672
- ORF length:
- 606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaggtgtcg gtgctgaacg tggcggtcct ggagaacccg agccctttcc 121 acagcccctt ccggttcgag atcagcttcg agtgcagtga agccctggcg gacgacctgg 181 agtggaagat catttatgtt ggctcggctg agagtgagga atttgatcag atcctagact 241 cggtgctggt gggccctgtg ccagcaggga gacacatgtt tgtctttcag gccGACGCCC 301 CCAACCCATC CCTCATCCCA GAGACTGATG CCGTGGGTGT GACTGTGGTC CTCATCACCT 361 GCACCTACCA TGGACAGGAG TTCATCCGAG TGGGCTACTA CGTCAACAAC GAGTACCTCA 421 ACCCTGAGCT GCGTGAGAAC CCGCCCATGA AGCCAGATTT CTCCCAGCTC CAGCGGAACA 481 TCTTGGCCTC GAACCCCCGG GTGACCCGCT TCCATATCAA CTGGGACAAC AACATGGACA 541 GGCTGGAGGC CATAGAGACC CAGGACCCCT CCCTGGGCTG CGGCCTCCCA CTCAACTGCA 601 CTCCTATCAA GGGCTTGGGG CTCCCTGGCT GCATCCCTGG CCTCCTCCCT GAGAACTCCA 661 TGGACTGCAT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 721 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 781 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTG ACAGTTCTTT ACTCCCTGGC 841 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t