Construct: ORF TRCN0000468692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012558.1_s317c1
- Derived from:
- ccsbBroadEn_11736
- DNA Barcode:
- TTGGAACTTTGTTTACTCGTGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SF3B3 (23450)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23450 | SF3B3 | splicing factor 3b subunit 3 | NM_012426.5 | 32.7% | 32.7% | 1_2454del;2696C>T |
| 2 | mouse | 101943 | Sf3b3 | splicing factor 3b, subunit 3 | XM_006530520.3 | 55.3% | 61% | (many diffs) |
| 3 | mouse | 101943 | Sf3b3 | splicing factor 3b, subunit 3 | XM_017312494.1 | 46.5% | 51.3% | (many diffs) |
| 4 | mouse | 101943 | Sf3b3 | splicing factor 3b, subunit 3 | NM_133953.2 | 29.5% | 32.6% | (many diffs) |
| 5 | mouse | 101943 | Sf3b3 | splicing factor 3b, subunit 3 | XM_006530518.3 | 29.5% | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaggaaatg gtggaagcag caggggagga tgagcgggag ctggccgcag 121 agatggcagc agcattcctc aatgaaaacc tccctgaatc catctttgga gctcccaagg 181 ctggcaatgg gcagtgggcc tctgtgatcc gagtgatgaa tcccattcaa gggaacacac 241 tggaccttgt ccagctggaa cagaatgagg cagcttttag tgtggctgtg tgcaggtttt 301 ccaacattgg tgaagactgg tatgtgctgg tgggtgtggc caaggacctg atactaaacc 361 cccgatctgt ggcagggggc ttcgtctata cttacaagct tgtgaacaat ggggaaaaac 421 tggagttttt gcacaagact cctgtggaag aggtccctgc tgctattgcc ccattccagg 481 ggagggtgtt gattggtgtg gggaagctgt tgcgtgtcta tgacctggga aagaagaagt 541 tactccgaaa atgtgagaat aagcatattg ccaattatat ctctgggatc cagactatcg 601 gacatagggt aattgtatct gatgtccaag aaagtttcat ctgggttcgc tacaagcgta 661 atgaaaacca gcttatcatc tttgctgatg atacctaccc ccgatgggtc actacagcca 721 gcctcctgga ctatgacact gtggctgggg cagacaagtt tggcaacata tgtgtggtga 781 ggctcccacc taacaccaat gatgaagtag atgaggatcc tacaggaaac aaagccctgt 841 gggaccgtgg cttgctcaat ggggccTCCC AGAAGGCAGA GGTGATCATG AACTACCATG 901 TCGGGGAGAC GGTGCTGTCC TTGCAGAAGA CCACGCTGAT CCCTGGAGGC TCAGAATCAC 961 TTGTCTATAC CACCTTGTCT GGAGGAATTG GCATCCTTGT GCCATTCACG TCCCATGAGG 1021 ACCATGACTT CTTCCAGCAT GTGGAAATGC ACCTGCGGTC TGAACATCCC CCTCTCTGTG 1081 GGCGGGACCA CCTCAGCTTT CGCTCCTACT ACTTCCCTGT GAAGAATGTG ATTGATGGAG 1141 ACCTCTGTGA GCAGTTCAAT TCCATGGAAC CCAACAAACA AAAGAACGTC TCTGAAGAAC 1201 TGGACCGAAC CCCACCCGAA GTGTCCAAGA AACTCGAGGA TATCCGGACC CGCTACGCCT 1261 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGATT GGAACTTTGT TTACTCGTGA CAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt