Construct: ORF TRCN0000468693
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007073.1_s317c1
- Derived from:
- ccsbBroadEn_06571
- DNA Barcode:
- TTTCCCACGTATCGACGTACTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCM6 (4175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468693
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4175 | MCM6 | minichromosome maintenance ... | NM_005915.6 | 99.8% | 99.7% | 937A>G;2040T>C;2446A>T |
| 2 | mouse | 17219 | Mcm6 | minichromosome maintenance ... | NM_008567.2 | 88.2% | 95.6% | (many diffs) |
| 3 | mouse | 17219 | Mcm6 | minichromosome maintenance ... | NM_001313695.1 | 85.2% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2529
- ORF length:
- 2463
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctcgcggcg gcagcggagc cgggcgccgg cagccagcac ctggaggtcc 121 gcgacgaggt ggccgagaag tgccagaaac tgttcctgga cttcttggag gagtttcaga 181 gcagcgatgg agaaattaaa tacttgcaat tagcagagga actgattcgt cctgagagaa 241 acacattggt tgtgagtttt gtggacctgg aacaatttaa ccagcaactt tccaccacca 301 ttcaagagga gttctataga gtttaccctt acctgtgtcg ggccttgaaa acattcgtca 361 aagaccgtaa agagatccct cttgccaagg atttttatgt tgcattccaa gacctgccta 421 ccagacacaa gattcgagag ctcacctcat ccagaattgg tttgctcact cgcatcagtg 481 ggcaggtggt gcggactcac ccagttcacc cagagcttgt gagcggaact tttctgtgct 541 tggactgtca gacagtgatc agggatgtag aacagcagtt caaatacaca cagccaaaca 601 tctgccgaaa tccagtttgt gccaacagga ggagattctt actggataca aataaatcaa 661 gatttgttga ttttcaaaag gttcgtattc aagagaccca agctgagctt cctcgaggga 721 gtatcccccg cagtttagaa gtaattttaa gggctgaagc tgtggaatca gctcaagctg 781 gtgacaagtg tgactttaca gggacactga ttgttgtgcc tgacgtctcc aagcttagca 841 caccaggagc acgtgcagaa actaattccc gtgtcagtgg tgttgatgga tatgagacag 901 aaggcattcg aggactccgg gcccttggtg ttagggacct ttcttatagg ctggtctttc 961 ttgcctgctg tgttgcgcca accaacccaa ggtttggggg ggaagagctc agagatgagg 1021 aacagacagc tgagagcatt aagaaccaaa tgactgtgaa agaatgggag aaagtgtttg 1081 agatgagtca agataaaaat ctataccaca atctttgtac cagcctgttc cctactatac 1141 atggcaatga tgaagtaaaa cggggtgtcc tgctgatgct ctttggtggc gttccaaaga 1201 caacaggaga agggacctct cttcgagggg acataaatgt ttgcattgtt ggtgacccaa 1261 gtacagctaa gagccaattt ctcaagcacg tggaggagtt cagccccaga gctgtctaca 1321 ccagtggtaa agcgtccagt gctgctggct taacagcagc tgttgtgaga gatgaagaat 1381 ctcatgagtt tgtcattgag gctggagctt tgatgttggc tgataatggt gtgtgttgta 1441 ttgatgaatt tgataagatg gacgtgcggg atcaagttgc tattcatgaa gctatggaac 1501 agcagaccat atccatcact aaagcaggag tgaaggctac tctgaacgcc cggacgtcca 1561 ttttggcagc agcaaaccca atcagtggac actatgacag atcaaaatca ttgaaacaga 1621 atataaattt gtcagctccc atcatgtccc gattcgatct cttctttatc cttgtggatg 1681 aatgtaatga ggttacagat tatgccattg ccaggcgcat agtagatttg cattcaagaa 1741 ttgaggaatc aattgatcgt gtctattccc tcgatgatat cagaagatat cttctctttg 1801 caagacagtt taaacccaag atttccaaag agtcagagga cttcattgtg gagcaatata 1861 aacatctccg ccagagagat ggttctggag tgaccaagtc ttcatggagg attacagtgc 1921 gacagcttga gagcatgatt cgtctctctg aagctatggc tcggatgcac tgctgtgatg 1981 aggtccaacc taaacatgtg aaggaagctt tccggttact gaataaatca atcatccgtg 2041 tggaaacacc tgatgtcaat ctagatcaag aggaagagat ccagatggag gtagatgagg 2101 gtgccggtgg catcaatggt catgctgaca gccctgcTCC TGTGAACGGG ATCAATGGCT 2161 ACAATGAAGA CATAAATCAA GAGTCTGCTC CCAAAGCCTC CTTAAGGCTG GGCTTCTCTG 2221 AGTACTGCCG AATCTCTAAC CTTATTGTGC TTCACCTCAG AAAGGTGGAA GAAGAAGAGG 2281 ACGAGTCAGC ATTAAAGAGG AGCGAGCTTG TTAACTGGTA CTTGAAGGAA ATCGAATCAG 2341 AGATAGACTC TGAAGAAGAA CTTATAAATA AAAAAAGAAT CATAGAGAAA GTTATTCATC 2401 GACTCACACA CTATGATCAT GTTCTAATTG AGCTCACCCA GGCTGGATTG AAAGGCTCCA 2461 CAGAGGGAAG TGAGAGCTAT GAAGAAGATC CCTACTTGGT AGTTAACCCT TACTACTTGC 2521 TCGAAGATTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2581 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2641 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTTCCC ACGTATCGAC GTACTCCTAC 2701 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt