Construct: ORF TRCN0000468704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003773.1_s317c1
- Derived from:
- ccsbBroadEn_11199
- DNA Barcode:
- CTGAATGCACCTTAAGTAGGCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPO (7173)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7173 | TPO | thyroid peroxidase | NM_175722.3 | 21.5% | 21.5% | (many diffs) |
| 2 | human | 7173 | TPO | thyroid peroxidase | XM_024453093.1 | 21.2% | 21.2% | (many diffs) |
| 3 | human | 7173 | TPO | thyroid peroxidase | XM_024453092.1 | 19.4% | 19.4% | (many diffs) |
| 4 | human | 7173 | TPO | thyroid peroxidase | NM_001206745.1 | 18.7% | 18.7% | (many diffs) |
| 5 | human | 7173 | TPO | thyroid peroxidase | NM_175719.3 | 18.7% | 18.7% | (many diffs) |
| 6 | human | 7173 | TPO | thyroid peroxidase | XM_024453091.1 | 18.5% | 18.4% | (many diffs) |
| 7 | human | 7173 | TPO | thyroid peroxidase | NM_175721.3 | 18.4% | 18.4% | (many diffs) |
| 8 | human | 7173 | TPO | thyroid peroxidase | XM_024453090.1 | 18.2% | 18.1% | (many diffs) |
| 9 | human | 7173 | TPO | thyroid peroxidase | NM_000547.5 | 17.6% | 17.5% | (many diffs) |
| 10 | human | 7173 | TPO | thyroid peroxidase | NM_001206744.2 | 17.6% | 17.5% | (many diffs) |
| 11 | human | 7173 | TPO | thyroid peroxidase | XM_024453087.1 | 17.5% | 17.5% | (many diffs) |
| 12 | human | 7173 | TPO | thyroid peroxidase | XM_024453088.1 | 17.5% | 17.5% | (many diffs) |
| 13 | human | 7173 | TPO | thyroid peroxidase | XM_024453089.1 | 17.5% | 17.5% | (many diffs) |
| 14 | human | 7173 | TPO | thyroid peroxidase | XM_011510380.3 | 17.4% | 17.4% | (many diffs) |
| 15 | human | 7173 | TPO | thyroid peroxidase | XM_024453086.1 | 17.4% | 17.3% | (many diffs) |
| 16 | human | 7173 | TPO | thyroid peroxidase | XM_024453085.1 | 17.3% | 17.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agcgctggct gtgctgtctg tcacgctggt tatggcctgc acagaagcct 121 tcttcccctt catctcgaga gggaaagaac tcctttgggg aaagccTGAG GAGTCTCGTG 181 TCTCTAGCGT CTTGGAGGAA AGCAAGCGCC TGGTGGACAC CGCCATGTAC GCCACGATGC 241 AGAGAAACCT CAAGAAAAGA GGAATCCTTT CTGCAGCTCA GCTTCTGTCT TTTTCCAAAC 301 TTCCTGAGCC AACAAGCGGA GTGATTGCCC GAGCAGCAGA GATAATGGAA ACATCAATAC 361 AAGCGATGAA AAGAAAAGTC AACCTGAAAA CTCAACAATC ACAGCATCCA ACGGATGCTT 421 TATCAGAAGA TCTGCTGAGC ATCATTGCAA ACATGTCTGG ATGTCTCCCT TACATGCTGC 481 CCCCAAAATG CCCAAACACT TGCCTGGCGA ACAAATACAG GCCCATCACA GGAGCTTGCA 541 ACAACAGAAA AATGAAATAT AAGCCCGACC ATTCCGAAAC TGCCAACTAC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGACTGAATG CACCTTAAGT AGGCCTTACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt