Construct: ORF TRCN0000468713
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002906.1_s317c1
- Derived from:
- ccsbBroadEn_02937
- DNA Barcode:
- GCCTTATTAATCTTCCATGACCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM50B (26240)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468713
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26240 | FAM50B | family with sequence simila... | NM_012135.3 | 100% | 100% | |
| 2 | human | 26240 | FAM50B | family with sequence simila... | XM_017010729.1 | 100% | 100% | |
| 3 | mouse | 108161 | Fam50b | family with sequence simila... | NM_138746.2 | 84.3% | 83.8% | (many diffs) |
| 4 | mouse | 108161 | Fam50b | family with sequence simila... | XM_006516526.1 | 84.3% | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagtacaag ggcaccatgc gcgaggcagg ccgtgccatg cacctcctca 121 agaagcgcga aaggcagcgg gagcagatgg aggtgctgaa gcagcgcatc gccgaggaga 181 ccatcctcaa gtcgcaggtg gacaagaggt tctcggcgca ttacgacgcc gtggaggccg 241 agctgaagtc cagcacggtg ggcctggtga ccctgaacga catgaaggcc cggcaggagg 301 ccctggtcag ggagcgcgag cggcagctgg ccaagcgcca gcacctggag gagcagcggc 361 tgcagcagga gcggcagcgg gagcaggagc agcggcgcga gcgcaagcgt aagatctcct 421 gcctgtcctt tgcactagac gacctcgatg accaggccga cgcggccgag gccaggcgcg 481 ccggaaacct gggcaagaac cccgacgtgg acaccagctt cctgccagac cgcgaccgcg 541 aggaggagga gaaccggctc cgagaggagc tgcgccaaga gtgggaggcg cagcgcgaga 601 aagtgaagga cgaggagatg gaggtcacct tcagctactg ggacggctcg ggccaccggc 661 gcacggtgcg ggtgcgcaag ggcaacacgg tgcagcagtt ccTGAAGAAG GCGCTGCAGG 721 GGCTGCGCAA GGACTTCCTG GAGCTGCGCT CCGCCGGCGT GGAGCAGCTC ATGTTCATCA 781 AGGAGGACCT CATCCTGCCG CACTACCACA CCTTCTACGA CTTCATCATC GCCAGGGCGA 841 GGGGCAAGAG CGGGCCGCTC TTCAGCTTCG ATGTGCACGA TGACGTGCGC CTGCTCAGCG 901 ACGCCACCAT GGAGAAGGAC GAGTCGCACG CGGGCAAGGT GGTGCTGCGC AGCTGGTACG 961 AGAAGAACAA GCACATCTTC CCCGCCAGCC GCTGGGAGGC CTATGACCCC GAGAAGAAGT 1021 GGGACAAGTA CACCATCCGC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCT TATTAATCTT 1201 CCATGACCTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt