Construct: ORF TRCN0000468732
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006961.1_s317c1
- Derived from:
- ccsbBroadEn_10606
- DNA Barcode:
- CCTAGCCGATCCCTCCAAGAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC285074 (285074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468732
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64795 | RMND5A | required for meiotic nuclea... | NM_022780.4 | 24.8% | 24.8% | (many diffs) |
| 2 | human | 285074 | LOC285074 | anaphase promoting complex ... | NR_026846.1 | 13.1% | 1_353del;526G>C;648_2225del | |
| 3 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_001355745.1 | 23.9% | 24.8% | (many diffs) |
| 4 | mouse | 68477 | Rmnd5a | required for meiotic nuclea... | NM_024288.2 | 23.9% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 384
- ORF length:
- 294
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGATCAGTG CGTGACGGTG GAGCGCGAGC 121 TGGAGAAGGT GCTGCACAAG TTCTCGGGCT ACGGGCAGCT GTGCGAGCGC GGCCTGGAGG 181 AGCTCATCGA CTACACCGGC GGTCTCAAGC ACGAGATCCT GCAGAGCCAC GGCCAAGATG 241 CTGAATTATC AGGGACACTT TCACTTGTTT TGACACAGTG CTGTAAAAGA ATAAAGGATA 301 CTGTTCAAAA ATTGGCCTCC GACCACAAAG ACATCCACAG CAGTGTTTCT CGGGTTGGAA 361 AAGCCATTGA TAAGGATTCA CTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 421 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 481 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTAGCCGATC 541 CCTCCAAGAA TATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 601 att