Construct: ORF TRCN0000468804
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007968.1_s317c1
- Derived from:
- ccsbBroadEn_05319
- DNA Barcode:
- GGCATGATTCGCAAACTCTATAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLCXD2 (257068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468804
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257068 | PLCXD2 | phosphatidylinositol specif... | NM_001185106.1 | 100% | 100% | |
| 2 | human | 257068 | PLCXD2 | phosphatidylinositol specif... | NM_153268.3 | 97.3% | 91.4% | (many diffs) |
| 3 | mouse | 433022 | Plcxd2 | phosphatidylinositol-specif... | NM_001134480.1 | 78% | 79.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 984
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctagcagtt aggaaggcca ggaggaaact caggatgggg accatctgct 121 cccccaaccc cagcgggaca aagacatcat cggaggtctg caatgccgac tggatggcct 181 cgctcccccc tcacctccac aacctccccc tttccaatct ggcaatccca ggctcacatg 241 attcattcag ctactgggtg gatgaaaagt ccccagtggg gcctgaccaa acccaagcta 301 tcaaacgcct cgccaggatc tccttggtga agaagctaat gaagaagtgg tctgtgactc 361 agaacctgac atttcgagaa cagctggaag ctgggatccg ctactttgac ctgcgtgtgt 421 cttccaaacc aggggatgcc gaccaggaga tctacttcat ccatgggctt tttggcatca 481 aggtctggga tgggctgatg gaaattgact cgtttcttac acagcacccc caggagatta 541 tcttcctgga tttcaaccac ttctatgcca tggatgagac ccatcacaaa tgcctggttc 601 tgcggatcca ggaggccttt ggaaacaagc tgtgcccagc ctgcagtgtg gaaagtttga 661 cgctgcgaac tctgtgggag aagaactgcc aggttcttat tttctaccac tgtcccTTCT 721 ACAAGCAGTA CCCCTTCCTG TGGCCAGGAA AGAAGATTCC AGCGCCCTGG GCAAACACCA 781 CAAGTGTGCG CAAACTAATC CTCTTCTTGG AGACCACTCT GAGTGAGCGG GCCTCACGGG 841 GCTCCTTCCA TGTCTCCCAA GCGATCCTCA CCCCCAGAGT GAAGACCATT GCCCGGGGCT 901 TGGTTGGGGG CCTCAAGAAC ACGCTGGTTC ATAGCAATCG CTGGAACAGC CATGGGCCAT 961 CCCTGCTGAG TCAGGAAAGA AGCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GCATGATTCG 1141 CAAACTCTAT AGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att