Construct: ORF TRCN0000468821
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003984.1_s317c1
- Derived from:
- ccsbBroadEn_12302
- DNA Barcode:
- ATTATGAACAACCGATGGCCAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRPV5 (56302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468821
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56302 | TRPV5 | transient receptor potentia... | NM_019841.7 | 52% | 51.4% | (many diffs) |
| 2 | human | 55503 | TRPV6 | transient receptor potentia... | NM_018646.6 | 40.8% | 38% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gggttttcta cctaaggcag aagggcccgg gagccaactc cagaaacttc 121 tgccctcctt tctggtcaga gaacaagact gggaccagca cctggacaag cttcatatgc 181 tgcagcagaa gaggattcta gagtctccac tgcttcgagc atccaaggaa aatgacctgt 241 ctgttcttag gcaacttcta ctggactgca cctgtgacgt tcgacaaaga ggagccctgg 301 gggagacggc gctgcacata gcagccctct atgacaactt ggaggcggcc ttggtgctga 361 tggaggctgc cccagagctg gtctttgagc ccaccacatg tgaggctttt gcaggtcaga 421 ctgcactgca catcgctgtt gtgaaccaga atgtgaacct ggtgcgtgcc ctgctcaccc 481 gcagggccag tgtctctgcc agagccacag gcactgcctt ccgccatagt ccccgcaacc 541 tcatctactt tggggagcac cctttgtcct ttgctgcctg tgtgaacagc gaggagatcg 601 tgcggctgct cattgagcat ggagctgaca tcagggccca ggactccctg ggaaacacag 661 tattacacat cctcatcctc cagcccaaca aaacctttgc ctgccagatg tacaacctgc 721 tgctgtccta tgatggacat ggggaccacc tgcagcccct ggaccttgtg cccaatcacc 781 agggtctcac ccccttcaag ctggctggag tggagggtaa cactgtgatg ttccagcacc 841 TGATGCAGAA GCGGAGGCAC ATCCAGTGGA CGTATGGACC CCTGACCTCC ATTCTCTACG 901 ACCTCACGGA GATCGACTCC TGGGGAGAGG AGCTGTCCTT CCTGGAGCTT GTGGTCTCCT 961 CTGATAAACG AGAGGCTCGC CAAATTCTGG AACAGACCCC AGTGAAGGAG CTGGTGAGCT 1021 TCAAGTGGAA CAAGTATGGC CGGCCGTACT TCTGCATCCT GGCTGCCTTG TACCTGCTCT 1081 ACATGATCTG CTTTACTACG TGCTGCGTCT ACCGCCCCCT TAAGTTTCGT GGTGGCAACC 1141 GCACTCATTC TCGAGACATC ACCATCCTCC AGCAAAAACT ACTACAGGTG ATTCTCCTCA 1201 GAAGGGGATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTATG AACAACCGAT GGCCAATCAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt