Construct: ORF TRCN0000468822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003311.1_s317c1
- Derived from:
- ccsbBroadEn_03346
- DNA Barcode:
- TGCATGGTCTAAAATCCACGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSG2 (51617)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51617 | NSG2 | neuronal vesicle traffickin... | NM_015980.5 | 100% | 100% | |
| 2 | mouse | 18197 | Nsg2 | neuron specific gene family... | NM_001290680.1 | 91% | 97% | (many diffs) |
| 3 | mouse | 18197 | Nsg2 | neuron specific gene family... | NM_001290681.1 | 91% | 97% | (many diffs) |
| 4 | mouse | 18197 | Nsg2 | neuron specific gene family... | NM_008741.4 | 91% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 579
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaagctgaac agtaacccca gcgagaaggg aaccaagccg ccttcagttg 121 aggatggctt ccagaccgtc cctctcatca ctcccttgga ggttaatcac ttacagctgc 181 ctgctccaga aaaggtGATT GTGAAGACAA GAACGGAATA TCAGCCGGAA CAGAAGAACA 241 AAGGGAAGTT CCGGGTGCCG AAAATCGCTG AATTTACGGT CACCATCCTT GTCAGCCTGG 301 CCCTAGCTTT CCTTGCGTGC ATCGTGTTCC TGGTGGTTTA CAAAGCCTTC ACCTATGATC 361 ACAGCTGCCC AGAGGGATTC GTCTATAAGC ACAAACGCTG TATCCCAGCC TCCCTGGATG 421 CTTACTACTC CTCCCAGGAC CCCAATTCCA GAAGCCGCTT CTACACAGTC ATCAGCCACT 481 ACAGCGTGGC CAAGCAGAGC ACTGCCCGGG CCATCGGGCC GTGGCTGTCA GCAGCCGCTG 541 TCATCCATGA GCCCAAGCCG CCCAAGACCC AGGGCCACTA CCCAACTTTC TTGTACAAAG 601 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 661 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 721 ACGATGCATG GTCTAAAATC CACGCGCGAC GCGTTAAGTC gacaatcaac ctctggatta 781 caaaatttgt gaaagatt