Construct: ORF TRCN0000468831
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007615.1_s317c1
- Derived from:
- ccsbBroadEn_13153
- DNA Barcode:
- AGTTTTTGTAGCATGAACATAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHH2 (130271)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468831
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XM_017003352.1 | 77.9% | 76.2% | (many diffs) |
| 2 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XR_001738621.1 | 69.4% | (many diffs) | |
| 3 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XR_001738620.1 | 68.8% | (many diffs) | |
| 4 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | NM_172069.4 | 52.8% | 51.7% | (many diffs) |
| 5 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XM_017003351.2 | 50.1% | 48.9% | (many diffs) |
| 6 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XM_024452695.1 | 48.5% | 47.3% | (many diffs) |
| 7 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XR_001738622.1 | 34.5% | (many diffs) | |
| 8 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XR_939657.2 | 34.1% | (many diffs) | |
| 9 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XR_426981.2 | 34% | (many diffs) | |
| 10 | human | 130271 | PLEKHH2 | pleckstrin homology, MyTH4 ... | XM_017003353.1 | 15.2% | 14% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2454
- ORF length:
- 2388
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagctttct gagccagagg gaccagtaga ttggaaggaa cgatgtgtag 121 ctctggagtc ccaactcatg aaatttagag ttcaagcaag caagatacga gagcttttag 181 cagagaagat gcaacagctt gagagacaag ttattgatgc tgaacgtcaa gcagaaaaag 241 cttttcaaca ggtacaagtt atggaagata aattaaaagc agctaatatt caaaccagtg 301 aatcagagac aagattatat aataagtgtc aagatctgga gtcgctaata caggaaaaag 361 atgacgtcat tcaaaacttg gaattgcaac ttgaagagca gaaacaaata agaatacaag 421 aagctaaaat aatagaagag aaagcagcta agataaaaga atgggtaaca gttaagttaa 481 atgagctgga attggagaat cagaatcttc gtttgatcaa ccaaaaccaa actgaagaga 541 taagaacaat gcagtcaaaa ctacaagaag ttcaaggaaa gaagtcatcc actgtctcta 601 cactaaagct ttcggaaggc cagcgcctga gcagtttgac ctttgggtgc tttttatctc 661 gagcaaggag tcctcctcaa gtagtaaaat ctgaggaaat gagcaagata tcatcgaaag 721 aacctgagtt cactgaagga aaagacatgg aagaaatgga aattccagaa aagtctgttg 781 ataaccaagt tctagaaaac aacagaggcc agagaacatt gcatcaaacc ccttgtggct 841 cagaacagaa tcggaaaaca agaacaagct ttgccacaga tggtggcatc tcccagaatt 901 ctggggctcc tgtgagtgac tggagctctg atgaggaaga cgggagcaaa ggaagatcca 961 agtccagatg cacatccacc ctctccagtc acacatctga ggaaggggtc cagtgtagca 1021 ggatgggaag tgaaatgtat ctgacagcat ctgatgacag cagctctata tttgaggaag 1081 agacttttgg cataaagaga ccagaacaca agaagctata ttcttggcag caggaggcac 1141 agtggaaagc tctaaatagt cctcttggaa agggaaattc tgaattaagt aaaaaggaac 1201 aagatagttc ctcggatgaa ctgaataaaa aatttcaatc ccagagactc gattattcat 1261 cttcatcgag tgaagccaac accccaagcc ctattttgac cccagcttta atgccaaagc 1321 atcctaactc actctctgga aaaggaacac aattagtgcc ttcatcacac ctgccacccc 1381 caaagttaag gattcctaat gttttcagta taagtgtagc actagccaaa aggcacttaa 1441 gccagccaca gttaagctct gacaggatgt ttggtacaaa tagaaacgct ataagcatga 1501 tacgaccact gagacctcag gaaactgatc ttgatctagt tgatggagac agtacagaag 1561 ttttagagaa tatggacacg agttgtgatg atggattatt ttcctatgac tccttggact 1621 ctccaaattc agatgaccag gaacactgtg actcagcaaa gaaggtggca tacagcaaac 1681 ctccaactcc tcccctgcac cgttttcctt cttgggaaag cagaatttat gctgtagcca 1741 aatcaggtat tcgaatgtct gaggccttca atatggagag tgttaataaa aattctgctg 1801 caaccctttc ctatactaca tcaggacttt atacatctct gatatacaag aacatgacca 1861 ccccagtgta tacaactttg aaggggaagg cgacccaaat aagtagcagc cctttcctgg 1921 atgactcatc tgggtcagag gaagaagaca gctccagatc cagctcccgg acgtcagagt 1981 cagactcacg cagtaggagt gggccaggca gccccagagc catgaaacga ggtgtgtctc 2041 tcTCCTCTGT GGCTTCTGAA AGTGATTATG CTATTCCTCC TGATGCTTAC TCCACAGACA 2101 CGGAGTACTC ACAGCCAGAG CAGAAGCTCC CAAAAACTTG CTCATCTTCC AGTGATAATG 2161 GGAAAAATGA ACCACTGGAA AAATCTGGTT ATTTATTAAA AATGAGTGGT AAAGTCAAGT 2221 CTTGGAAGCG GCGGTGGTTT GTTCTTAAAG GTGGTGAATT ACTTTACTAC AAATCTCCGA 2281 GTGATGTAAT TAGAAAACCC CAGGGCCATA TTGAACTTAG TGCATCCTGT AGTATTTTAA 2341 GAGGAGATAA CAAACAAACA GTTCAGGTAC TTAACTTTTT TTTTTTTTTT TTTTTTTTTG 2401 TATCATGCCA GACTCAATTC TCAATTATCC AACCTAATGG AAAGGAGATA GGATACCCAA 2461 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2521 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2581 TATCTTGTGG AAAGGACGAA GTTTTTGTAG CATGAACATA AGCACGCGTT AAGTCgacaa 2641 tcaacctctg gattacaaaa tttgtgaaag att