Construct: ORF TRCN0000468835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005366.1_s317c1
- Derived from:
- ccsbBroadEn_12001
- DNA Barcode:
- GGCCAGACATCGATCTACGTGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPH5 (51611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468835
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_005270939.5 | 26.7% | 26.7% | 1_378del |
| 2 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_011541570.3 | 26.7% | 26.7% | 1_378del |
| 3 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_017001461.2 | 26.7% | 26.7% | 1_378del |
| 4 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_001077395.2 | 16.1% | 16.1% | 1_714del |
| 5 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_001077394.1 | 16.1% | 16.1% | 1_717del |
| 6 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | NM_015958.3 | 16.1% | 16.1% | 1_717del |
| 7 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_017001460.2 | 16.1% | 16.1% | 1_717del |
| 8 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_005270938.2 | 14.2% | 1.8% | 1_613del;720_721ins31 |
| 9 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_024447600.1 | 14.2% | 1.8% | 1_613del;720_721ins31 |
| 10 | human | 51611 | DPH5 | diphthamide biosynthesis 5 | XM_024447601.1 | 14.2% | 1.8% | 1_613del;720_721ins31 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 296
- ORF end:
- 434
- ORF length:
- 138
- Sequence:
-
1 TCCGCTCCCG GCGGATTTGT CCTACTCAGG AGAGCGTTCA CCGACAAACA ACAGATAAAA 61 CGAAAGGCCC AGTCTTCCGA CTGAGCCTTT CGTTTTATTT GATGCCTGGC AGTTCCCTAC 121 TCTCGCGTTA ACGCTAGCAT GGATGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG 181 TCTTAAGCTC GGGCCCCAAA TAATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA 241 ACAAATTGAT GAGCAATGCT TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCATGTG 301 CACTGTGGAC TTGGGAGAAC CATTGCATTC CTTGATCATC ACAGGAGGCA GCATACATCC 361 AATGGAGATG GAGATGCTAA GTCTGTTTTC CATACCAGAA AATAGCTCAG AATCTCAAAG 421 CATCAATGGA CTTTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 481 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 541 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GCCAGACATC GATCTACGTG 601 TAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att