Construct: ORF TRCN0000468859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003536.1_s317c1
- Derived from:
- ccsbBroadEn_04610
- DNA Barcode:
- GCCAGCCCCGGATATGAGTTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468859
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178191.2 | 100% | 100% | |
| 2 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_178190.3 | 84% | 84.5% | 180_213delinsG |
| 3 | human | 93974 | ATP5IF1 | ATP synthase inhibitory fac... | NM_016311.5 | 56.2% | 56.6% | 180_318delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 285
- ORF end:
- 465
- ORF length:
- 180
- Sequence:
-
1 GGCGCCGTCC AGGCACCTCG ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG 61 GGGGGAGGGG TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG 121 GCCAGCTTGG CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG 181 GTTCATTCTC AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG 241 AGGCTAGCAT CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCATGGCA GTGACGGCGT 301 TGGCGGCGCG GACGTGGCTT GGCGTGTGGG GCGTGAGGAC CATGCAAGCC CGAGGCTTCG 361 GCTCGGATCA GTCCGAGAAT GTCGACCGGG GCGCGGGCTC CATCCGGGAA GCCGGTGGGG 421 CCTTCGGAAA GAGAGAGCAG GCTGAAGAGG AACGATATTT CCGGTGCCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA GCCAGCCCCG GATATGAGTT TTTTACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt