Construct: ORF TRCN0000468866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011998.1_s317c1
- Derived from:
- ccsbBroadEn_04529
- DNA Barcode:
- CTTGTTGCCGTGCCGTCGCTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAGE5 (90737)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468866
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90737 | PAGE5 | PAGE family member 5 | NM_130467.5 | 100% | 100% | |
| 2 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530785.2 | 85.1% | 73.9% | (many diffs) |
| 3 | human | 90737 | PAGE5 | PAGE family member 5 | NM_001013435.3 | 84.6% | 84.6% | 0_1ins60 |
| 4 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530786.3 | 82.7% | 75.5% | (many diffs) |
| 5 | human | 389860 | PAGE2B | PAGE family member 2B | NM_001015038.3 | 80.1% | 74.8% | (many diffs) |
| 6 | human | 389860 | PAGE2B | PAGE family member 2B | XM_011530787.2 | 80.1% | 74.8% | (many diffs) |
| 7 | human | 389860 | PAGE2B | PAGE family member 2B | XM_017029513.1 | 80.1% | 74.8% | (many diffs) |
| 8 | human | 203569 | PAGE2 | PAGE family member 2 | NM_207339.4 | 77.8% | 69.4% | (many diffs) |
| 9 | human | 203569 | PAGE2 | PAGE family member 2 | XM_017029353.1 | 77.8% | 69.4% | (many diffs) |
| 10 | human | 100421746 | LOC100421746 | PAGE family member 5 pseudo... | NR_130179.1 | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA GGCGCCATGG GCCGGTAATC GTGGCTGGGC TGGAACGAGG GAGGAAGTGA 121 GAGATATGAG TGAGCATGTA ACAAGATCCC AATCCTCAGA AAGAGGAAAT GACCAAGAGT 181 CTTCCCAGCC AGTTGGACCT GTGATTGTCC AGCAGCCCAC TGAGGAAAAA CGTCAAGAAG 241 AGGAACCACC AACTGATAAT CAGGGTATTG CACCTAGTGG GGAGATCAAA AATGAAGGAG 301 CACCTGCTGT TCAAGGGACT GATGTGGAAG CTTTTCAACA GGAACTGGCT CTGCTTAAGA 361 TAGAGGATGC ACCTGGAGAT GGTCCTGATG TCAGGGAGGG GACTCTGCCC ACTTTTGATC 421 CCACTAAAGT GCTGGAAGCA GGTGAAGGGC AACTATACCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ACTTGTTGCC GTGCCGTCGC TGGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt