Construct: ORF TRCN0000468871
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004062.1_s317c1
- Derived from:
- BRDN0000395097
- DNA Barcode:
- GCGGGCTTCACACATAGGTTTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RLF (6018)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468871
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6018 | RLF | rearranged L-myc fusion | NM_012421.4 | 99.9% | 99.9% | 4935T>G |
| 2 | human | 6018 | RLF | rearranged L-myc fusion | XM_017002016.2 | 88.6% | 88.6% | 0_1ins648;4287T>G |
| 3 | human | 6018 | RLF | rearranged L-myc fusion | XM_017002017.2 | 83.5% | 83.4% | 0_1ins937;2_3insTGTTCTTG;3990T>G |
| 4 | human | 6018 | RLF | rearranged L-myc fusion | XM_017002018.2 | 79.6% | 79.6% | 0_1ins1167;3768T>G |
| 5 | mouse | 109263 | Rlf | rearranged L-myc fusion seq... | NM_001346660.1 | 86.3% | 87.9% | (many diffs) |
| 6 | mouse | 109263 | Rlf | rearranged L-myc fusion seq... | XM_006502657.4 | 76.2% | 77.4% | (many diffs) |
| 7 | mouse | 109263 | Rlf | rearranged L-myc fusion seq... | XM_006502658.2 | 76.2% | 77.4% | (many diffs) |
| 8 | mouse | 109263 | Rlf | rearranged L-myc fusion seq... | XM_017319915.2 | 76.2% | 77.4% | (many diffs) |
| 9 | mouse | 109263 | Rlf | rearranged L-myc fusion seq... | XM_030253080.1 | 76.2% | 77.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5811
- ORF length:
- 5742
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacgga aagggagacg ccgccgctgt cgccggggct ggggctgagg 121 ctccggcggt agcgggagcc ggagatggag tcgagactga gtccatggtt cggggtcatc 181 gccccgtatc tccagcgccg ggagcctcgg gactgcggcc gtgtctgtgg cagctggaga 241 cagagctgag ggagcaagag gtgtcggagg tctcatcttt gaactactgc cggagcttct 301 gccagacctt attgcaatat gcaagcaaca agaatgcatc agaacatatt gtgtatcttc 361 tggaggtata tcgacttgcc atccaaagct ttgccagtgc acgtccatac ttaactactg 421 aatgtgaaga tgtcctctta gtgcttggca gattagtact gagttgtttc gaattactgc 481 tttcagtgtc tgaaagtgaa ctgccatgtg aagtctggct accattcctt cagtctctac 541 aggagtcaca tgatgcatta ttggaatttg ggaataataa cctacaaata ttggttcatg 601 ttaccaagga aggggtgtgg aaaaacccag ttcttcttaa aattctgtct caacagccag 661 tagaaacgga ggaagtcaat aaattgattg cacaagaagg accttccttt ctgcaaatgc 721 gaataaaaca tttgttgaaa tctaactgca tcccccaggc tactgcttta tcaaaactat 781 gtgcagaatc taaagaaatt tcaaatgtgt catcttttca gcaagcctat atcacatgtt 841 tatgttctat gctccctaat gaagatgcta ttaaggagat tgcaaaggtc gactgcaagg 901 aagtactaga catcatttgt aatctggaat ctgaggggca ggataacaca gcatttgttc 961 tttgtacgac ttaccttacc cagcagctcc aaactgcaag tgtatattgt tcttgggaac 1021 tgactctttt ttggagtaaa ctgcaaagaa gaattgaccc ttctttagat acttttttgg 1081 agcgctgtcg tcagtttggt gtcatagcta aaacgcagca gcatttattt tgcctcatta 1141 gagttataca aactgaagca caagatgctg gtcttggggt gtcaatttta ctgtgtgtca 1201 gagctcttca actcagatca agtgaagatg aggaaatgaa ggcatcagtt tgtaaaacaa 1261 ttgcctgtct tttaccagaa gatttagaag ttagacgagc ctgtcagctt acagaattct 1321 taattgaacc cagtttggat ggatttaata tgttagaaga actatatttg caaccagatc 1381 aaaaatttga tgaagaaaat gcaccggttc caaattctct tcgatgtgag ctcttactag 1441 ctttaaaagc ccactggcct tttgatcctg agttttggga ctggaaaact ttaaaacgac 1501 actgccacca acttttagga caagaagcct cagattctga tgatgattta agtggctatg 1561 aaatgtccat taatgacaca gatgttttag agtcatttct cagtgactat gatgagggta 1621 aagaagataa acaatataga agaagagatt tgacagatca gcataaggag aaaagagaca 1681 aaaaacctat tggctcttct gaaagatatc agaggtggct tcagtacaag tttttctgtt 1741 tgttatgtaa gcgggaatgt atagaggcta gaattcttca tcattctaag atgcatatgg 1801 aagatggaat ttacacctgt ccagtttgta ttaaaaaatt taagagaaaa gaaatgtttg 1861 ttcctcatgt gatggagcat gttaaaatgc caccaagcag aagggaccgc tctaaaaaga 1921 aattactgtt aaaaggctct caaaagggta tttgtcctaa gagcccctct gcaatcccag 1981 agcaaaacca ttcattgaat gaccaagcca aaggagagtc tcatgaatat gtcacattca 2041 gcaaattaga agattgccac ctgcaagaca gagatttgta tccatgtccc ggtacagact 2101 gttcccgtgt gtttaagcaa tttaaatact taagtgtgca tcttaaagct gaacaccaaa 2161 ataatgatga aaatgccaag cactacttgg atatgaaaaa tagaagagag aagtgtactt 2221 actgtcgacg acattttatg tctgcttttc accttcgaga gcacgaacaa gtgcattgtg 2281 ggcctcagcc ttatatgtgt gtatctatag attgctatgc taggtttgga tcagtaaatg 2341 aactacttaa ccataaacaa aagcatgacg atctgcgtta caaatgtgaa ttaaatggct 2401 gtaatattgt tttcagtgac ttgggacagc tttaccacca tgaagcacaa cactttaggg 2461 atgcatctta cacatgcaac ttccttggct gtaaaaagtt ctattactcc aaaattgaat 2521 accagaatca cctctcaatg cataatgttg aaaattcaaa tggagacata aagaaatcag 2581 tgaaacttga ggagtctgca acaggtgaaa agcaagattg tattaatcag ccccatctac 2641 ttaaccaaac tgataaatca catttacctg aagatctttt ctgtgcagaa tcagctaatt 2701 ctcaaataga tacagaaact gcagaaaacc tgaaagaaaa cagtgacagt aattctagtg 2761 atcagttaag tcatagctct tcagcttcaa tgaatgaaga gctaattgac acactagatc 2821 actctgaaac tatgcaggat gtattgttat ctaatgagaa agtctttggg ccctccagtt 2881 taaaagaaaa atgttccagt atggcagttt gttttgacgg gactaagttt acctgtggtt 2941 ttgatggctg tggttccaca tacaaaaatg caagaggaat gcagaaacat ttacggaagg 3001 ttcatccata ccatttcaag cccaaaaaga taaagacgaa agatctgttt ccctctttgg 3061 gtaatgaaca taatcagaca actgaaaagt tggatgcaga acctaaaccc tgctcagata 3121 caaacagtga ctccccagat gaaggtctag atcacaatat tcacattaaa tgtaaacgag 3181 aacatcaagg ttattcctca gaatcctcca tttgtgcttc taaaaggccc tgtacagagg 3241 ataccatgtt ggaacttctg ttacgcttga aacatttaag cttgaaaaac tcaataacac 3301 atggatcttt ctcagggtca ttgcaggggt acccatccag tggtgctaag tctcttcagt 3361 cagtttcatc tatctcagac cttaattttc agaatcaaga tgaaaacatg ccaagtcagt 3421 accttgcaca gttggcggct aagccgtttt tctgtgagct tcaaggatgc aaatatgaat 3481 ttgtgaccag agaggctctg ttaatgcatt atcttaaaaa gcataattat tcaaaagaaa 3541 aagtccttca gttaaccatg ttccaacatc ggtattcccc atttcagtgt catatttgcc 3601 aaaggtcatt tacaagaaaa acacacctta ggattcatta taaaaataaa catcaaattg 3661 gcagtgacag agcaactcac aaactattag ataatgaaaa gtgtgatcat gaaggcccat 3721 gttcagtaga taggttgaaa ggtgattgtt ctgcagaact tggaggtgat cccagtagta 3781 actctgagaa accacactgt catcctaaaa aggatgaatg tagttctgaa acagatttgg 3841 aatcatcttg tgaagaaaca gaaagtaaaa catctgacat ttcatcacca ataggcagcc 3901 atagagaaga acaagaagga agagagggca gaggtagcag gcgaactgtt gctaaaggaa 3961 atctgtgtta tattttgaat aaataccaca aaccattcca ttgtattcat aaaacttgca 4021 actcctcatt caccaatcta aaaggcttaa ttcgccatta cagaactgta catcagtaca 4081 acaaagaaca gttatgtttg gagaaagaca aagcaagaac caaaagggaa cttgtcaaat 4141 gtaaaaagat atttgcttgc aaatataagg aatgtaataa acgcttcctg tgttccaaag 4201 ctcttgctaa gcactgtagt gattctcata acctagacca tattgaagag cctaaagtac 4261 tttccgaagc tggatctgca gcaaggtttt cttgtaacca gcctcagtgc cctgctgttt 4321 tttatacatt caacaagttg aagcaccact tgatggaaca gcataatatt gaaggggaaa 4381 tacattcaga ttatgaaatt cattgtgatc ttaatggctg tggccagatt ttcacccatc 4441 gcagtaatta ctcacaacat gtatattacc gacataaaga ctattatgat gatttgttta 4501 gaagccagaa agtagcaaat gagagactac taaggagtga aaaggtatgt caaacagctg 4561 atactcaggg gcatgaacat cagaccacca ggagatcatt taatgctaag tctaaaaaat 4621 gtggcttaat caaagaaaag aaagccccaa taagttttaa aaccagagct gaggccctcc 4681 atatgtgtgt ggagcactct gagcacacac agtacccctg catggttcaa ggatgcttat 4741 ctgtggtgaa gttggagagc agcattgtga ggcattacaa acgcactcat cagatgagta 4801 gtgcctattt agagcaacag atggagaatc ttgttgtttg cgttaagtac ggtaccaaaa 4861 ttaaggagga acccccttct gaagcagatc cctgtataaa gaaagaagaa aatagaagct 4921 gtgaatcaga gcgcacagaa cacagccatt ccccgggtga cagtagtgca cccatccaga 4981 acactgattg ctgtcattca agggaaaggg atggaggtca gaaagggtgc atagaaagca 5041 gctcagtatt tgatgcagat actctgctct acaggggaac tttgaaatgt aatcatagtt 5101 ccaaaaccac ttccctagaa cagtgtaata tagttcagcc tcctcctcct tgtaaaatag 5161 aaaattccat acctaatccc aatgggactg aaagtgggac ttatttcaca agtttccagc 5221 tgcctttacc aaggatcaaa gaatcagaaa ctaggcagca tagttcaggg caagaaaaca 5281 ctgtaaaaaa tccaacccat gtcccaaaag agaattttag gaaacattca cagccccggt 5341 catttgattt gaagacttac aaacctatgG GATTTGAATC TTCATTTCTG AAATTTATTC 5401 AGGAAAGTGA AGAGAAAGAA GATGATTTTG ATGATTGGGA GCCTTCAGAG CACTTAACAT 5461 TAAGTAATTC TTCACAGTCC AGTAATGATT TAACAGGGAA TGTTGTGGCA AATAATATGG 5521 TGAATGACAG TGAACCTGAA GTTGACATAC CTCATTCTTC CAGTGACTCT ACAATTCATG 5581 AGAACCTGAC TGCAATCCCA CCTTTAATAG TAGCTGAAAC AACAACAGTT CCTTCCTTGG 5641 AAAACCTGAG GGTTGTATTG GACAAAGCAT TAACAGACTG TGGAGAGCTT GCCTTAAAAC 5701 AGCTTCATTA TCTTCGGCCA GTGGTGGTTC TTGAAAGATC TAAGTTTTCC ACACCAATTT 5761 TAGACTTATT TCCAACAAAA AAGACAGATG AGCTTTGTGT AGGAAGTTCA TTGCCAACTT 5821 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 5881 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 5941 CTTGTGGAAA GGACGAGCGG GCTTCACACA TAGGTTTACT ACGCGTTAAG TCgacaatca 6001 acctctggat tacaaaattt gtgaaagatt