Construct: ORF TRCN0000468872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001149.1_s317c1
- Derived from:
- ccsbBroadEn_11939
- DNA Barcode:
- GTAGGGGCACATGCTACCCGTTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM135B (51059)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51059 | FAM135B | family with sequence simila... | XM_011517074.1 | 35.5% | 35.4% | (many diffs) |
| 2 | human | 51059 | FAM135B | family with sequence simila... | XM_011517073.2 | 35% | 35% | (many diffs) |
| 3 | human | 51059 | FAM135B | family with sequence simila... | XM_011517072.2 | 34.1% | 34% | (many diffs) |
| 4 | human | 51059 | FAM135B | family with sequence simila... | XM_011517070.2 | 33.9% | 33.9% | 1_2400del;2526C>T;3280_3291del |
| 5 | human | 51059 | FAM135B | family with sequence simila... | XM_011517069.2 | 32% | 31.9% | (many diffs) |
| 6 | human | 51059 | FAM135B | family with sequence simila... | XM_011517068.2 | 31.5% | 31.5% | 1_2103del;2229C>T;3342_3921delinsG |
| 7 | human | 51059 | FAM135B | family with sequence simila... | XM_017013471.1 | 29.6% | 29.6% | (many diffs) |
| 8 | human | 51059 | FAM135B | family with sequence simila... | XM_011517065.1 | 29.5% | 29.5% | (many diffs) |
| 9 | human | 51059 | FAM135B | family with sequence simila... | NM_001362965.1 | 29.3% | 29.3% | 1_2400del;2526C>T;3639_4218delinsG |
| 10 | human | 51059 | FAM135B | family with sequence simila... | NM_015912.4 | 29.3% | 29.3% | 1_2400del;2526C>T;3639_4218delinsG |
| 11 | human | 51059 | FAM135B | family with sequence simila... | XM_011517064.2 | 29.3% | 29.3% | 1_2400del;2526C>T;3639_4218delinsG |
| 12 | human | 51059 | FAM135B | family with sequence simila... | XM_011517056.2 | 29.2% | 29.2% | (many diffs) |
| 13 | human | 51059 | FAM135B | family with sequence simila... | XM_011517058.2 | 29.2% | 29.2% | (many diffs) |
| 14 | human | 51059 | FAM135B | family with sequence simila... | XM_011517059.1 | 29.2% | 29.2% | (many diffs) |
| 15 | human | 51059 | FAM135B | family with sequence simila... | XM_011517060.2 | 29.2% | 29.2% | (many diffs) |
| 16 | human | 51059 | FAM135B | family with sequence simila... | XM_011517061.2 | 29.2% | 29.2% | (many diffs) |
| 17 | human | 51059 | FAM135B | family with sequence simila... | XM_011517062.1 | 29.2% | 29.2% | (many diffs) |
| 18 | human | 51059 | FAM135B | family with sequence simila... | XM_011517063.2 | 29.2% | 29.2% | (many diffs) |
| 19 | human | 51059 | FAM135B | family with sequence simila... | XR_001745532.1 | 16.1% | 1_3380del;3506C>T;4620_7684del | |
| 20 | human | 51059 | FAM135B | family with sequence simila... | XR_001745531.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1308
- ORF length:
- 1239
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacagcaag agccaaggtt ccccaggatc ttgctctcaa ctttgtggtg 121 actctggaac agatgctgga gcagaccatc ccctggtgga gatagtttta gatgctgaca 181 accagcaggg ccctggatac atagacatcc ccaaagggaa agggaagcag tttgatgctc 241 aaggacactg tcttcctgat ggcaggactg agaacactcc aggtgttgaa accaaaggtc 301 ttaatttaaa aataccacgc gtcatagcac ttgaaaaccc caggaccaga tctcttcata 361 gagcacttga ggaaacccca aagggcatgc ctaaagactt gaatgtgggt cagcaagctc 421 tttccaacag tggcatctca gaggttgagg gtctctctca acatcaggtg cctgaattga 481 gctgtacgtc agctgctgat gccatcaaca ggaactcaac aggccagcaa agccaaagcg 541 gttcaccttg cattatggat gacacagcat ttaatagagg agtgaatgcc ttcccggagg 601 ctaaacataa agcaggcact gtgtgcccca ctgtgaccca ttccgttcat tcccaggttt 661 tgaaaaacca agagctgaag gcaggcactt ccatcatggg gtcccatctg acttctgcag 721 agacctttac tctggacagc ctgaaggctg tggaggttgt gaacttatct gtgtcttgca 781 ctgccacctg tctccctttc tcatctgtgc ccaaggagac ccctgccagg gctggattct 841 cttccaaaca gaccctgttt cccatcaccc atcagccttt gggatccttt GGAGTTGTTT 901 CTACCCATTC CAGCACGTTG GATGAGGAAG TCAGTGAGAG GATGTTTAGT TTTTATCAGG 961 CCAAAGAAAA ATTTAAAAAA GAACTGAAGA TTGAAGGATT TCTGTACAGT GACTTAACTG 1021 TACTAGCTTC TGATATACCA TATTTCCCAC CAGAGGAAGA GGAAGAAAAT TTGGAAGATG 1081 GAATTCACCT GGTTGTCTGT GTCCATGGCC TGGATGGGAA CAGTGCAGAC CTCCGGCTGG 1141 TAAAGACTTT CATAGAACTG GGGCTCCCTG GAGGAAAACT GGACTTCCTA ATGTCTGAAA 1201 AGAATCAGAT GGACACATTT GCAGATTTTG ATACTATGAC GGATCGGTTA TTGGATGAAA 1261 TCATTCAACA CATTCAGTTG TACAACCTCT CCATATCCCG AATTAGGTTG CCAACTTTCT 1321 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1381 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1441 GTGGAAAGGA CGAGTAGGGG CACATGCTAC CCGTTCGACG CGTTAAGTCg acaatcaacc 1501 tctggattac aaaatttgtg aaagatt