Construct: ORF TRCN0000468877
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012797.1_s317c1
- Derived from:
- ccsbBroadEn_11088
- DNA Barcode:
- ATCACCCGGGTTCTCTGTTACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD51D (5892)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468877
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5892 | RAD51D | RAD51 paralog D | NM_133629.3 | 83.7% | 80.1% | (many diffs) |
| 2 | human | 5892 | RAD51D | RAD51 paralog D | NM_002878.3 | 63.7% | 63.7% | 1_357del |
| 3 | human | 5892 | RAD51D | RAD51 paralog D | NM_001142571.2 | 60% | 60% | 1_417del |
| 4 | human | 100529207 | RAD51L3-RFFL | RAD51L3-RFFL readthrough | NR_037714.1 | 14.2% | (many diffs) | |
| 5 | human | 5892 | RAD51D | RAD51 paralog D | NR_037711.2 | 6.4% | 1_383del;1011_9745del | |
| 6 | human | 5892 | RAD51D | RAD51 paralog D | NR_037712.2 | 6.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agcaaatgtg gcccatggcc tgcagcaaaa cgtcctatat gtagattcca 121 atggagggct gacagcttcc cgcctcctcc agctgcttca ggctaaaacc caggatgagg 181 aggaacaggc agaagctctc cggaggatcc aggtggtgca tgcatttgac atcttccaga 241 tgctggatgt gctgcaggag cTCCGAGGCA CTGTGGCCCA GCAGGTGACT GGTTCTTCAG 301 GAACTGTGAA GGTGGTGGTT GTGGACTCGG TCACTGCGGT GGTTTCCCCA CTTCTGGGAG 361 GTCAGCAGAG GGAAGGCTTG GCCTTGATGA TGCAGCTGGC CCGAGAGCTG AAGACCCTGG 421 CCCGGGACCT TGGCATGGCA GTGGTGGTGA CCAACCACAT AACTCGAGAC AGGGACAGCG 481 GGAGGCTCAA ACCTGCCCTC GGACGCTCCT GGAGCTTTGT GCCCAGCACT CGGATTCTCC 541 TGGACACCAT CGAGGGAGCA GGAGCATCAG GCGGCCGGCG CATGGCGTGT CTGGCCAAAT 601 CTTCCCGACA GCCAACAGGT TTCCAGGAGA TGGTAGACAT TGGGACCTGG GGGACCTCAG 661 AGCAGAGTGC CACATTACAG GGTGATCAGA CATGCCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 841 CACCCGGGTT CTCTGTTACT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt