Transcript: Human NM_133629.3

Homo sapiens RAD51 paralog D (RAD51D), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
RAD51D (5892)
Length:
9528
CDS:
146..796

Additional Resources:

NCBI RefSeq record:
NM_133629.3
NBCI Gene record:
RAD51D (5892)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_133629.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000154018 CGTGTATGTTTCTGGTGGAAA pLKO.1 1044 3UTR 100% 4.950 3.960 N RAD51D n/a
2 TRCN0000153705 CCACGTTTCCTTCCCTTATTT pLKO.1 1019 3UTR 100% 15.000 10.500 N RAD51D n/a
3 TRCN0000151019 GAAATGTGGCTTGTCTTACAA pLKO.1 268 CDS 100% 5.625 3.938 N RAD51D n/a
4 TRCN0000158392 CTGGAAGAGGTAGCTCAGAAA pLKO.1 251 CDS 100% 4.950 3.465 N RAD51D n/a
5 TRCN0000158391 CCTGTGCTGTTGTTTGGGAAA pLKO.1 797 CDS 100% 4.050 2.835 N RAD51D n/a
6 TRCN0000430764 GGTTTCCAGGAGATGGTAGAC pLKO_005 719 CDS 100% 4.050 2.835 N RAD51D n/a
7 TRCN0000157052 GCTCAGAAATGTGGCTTGTCT pLKO.1 263 CDS 100% 3.000 2.100 N RAD51D n/a
8 TRCN0000429115 ATCCAGGTGGTGCATGCATTT pLKO_005 308 CDS 100% 10.800 5.400 Y RAD51D n/a
9 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3063 3UTR 100% 4.950 2.475 Y ERAP2 n/a
10 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 3131 3UTR 100% 13.200 6.600 Y IQCC n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3064 3UTR 100% 13.200 6.600 Y LIAS n/a
12 TRCN0000052651 GCTGGAGCCACTGAAAGAAAT pLKO.1 6753 3UTR 100% 13.200 6.600 Y MOB3A n/a
13 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2792 3UTR 100% 4.950 2.475 Y DCAF11 n/a
14 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 4995 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133629.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11088 pDONR223 100% 83.7% 80.1% None (many diffs) n/a
2 ccsbBroad304_11088 pLX_304 0% 83.7% 80.1% V5 (many diffs) n/a
3 TRCN0000468877 ATCACCCGGGTTCTCTGTTACTCT pLX_317 68.3% 83.7% 80.1% V5 (many diffs) n/a
4 ccsbBroadEn_01370 pDONR223 100% 65.8% 65.8% None 142_143ins336 n/a
5 ccsbBroad304_01370 pLX_304 0% 65.8% 65.8% V5 142_143ins336 n/a
6 TRCN0000466966 GACGGAGTTGGTCTGACTGTAAAT pLX_317 35.3% 65.8% 65.8% V5 142_143ins336 n/a
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