Construct: ORF TRCN0000468898
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004407.3_s317c1
- Derived from:
- ccsbBroadEn_15121
- DNA Barcode:
- TAACTAATTTCGTCTTAACAAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCYL3 (57147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468898
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | NM_020423.7 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001862.1 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001863.1 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | NM_181093.3 | 92.5% | 92.5% | (many diffs) |
| 5 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_006711465.1 | 92.5% | 92.5% | (many diffs) |
| 6 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509801.1 | 92.5% | 92.5% | (many diffs) |
| 7 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001864.1 | 79.1% | 79.2% | (many diffs) |
| 8 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_017001865.1 | 79.1% | 79.2% | (many diffs) |
| 9 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509802.1 | 73.4% | 73.4% | (many diffs) |
| 10 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_011509803.1 | 73.4% | 73.4% | (many diffs) |
| 11 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XR_001737336.2 | 69.8% | (many diffs) | |
| 12 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XR_001737335.2 | 54.4% | (many diffs) | |
| 13 | human | 57147 | SCYL3 | SCY1 like pseudokinase 3 | XM_024448565.1 | 44.8% | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2130
- ORF length:
- 2064
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg atcagagaac agtgctttaa agagctatac actgagagaa ccaccattta 121 ccttaccctc tggacttgct gtttatcccg ctgtactgca agatggcaaa tttgcttcag 181 tttttgtgta taagagagaa aatgaagaca aggttaataa agctgccaag catttgaaga 241 cacttcgtca cccttgcttg ctaagatttt tatcttgtac tgtggaagcg gatggcattc 301 atcttgtcac tgagcgagta cagcccctgg aagtggcttt ggaaacattg tcttctgcag 361 aggtctgtgc tgggatctat gacatattgc tggctcttat cttccttcat gacagaggac 421 acctaacaca caataatgtc tgtttatcat ctgtgtttgt gagtgaagat ggacactgga 481 agctaggagg aatggaaact gtttgtaaag tttctcaggc cacaccagag tttctgagga 541 gtattcagtc aataagagac ccagcatcta tccctcctga agagatgtct ccagaattca 601 caactctccc agagtgtcat ggacatgccc gggatgcctt ttcatttgga acattggtgg 661 aaagtttgct cacaatctta aatgaacagg tttcagcgga tgttctctcc agctttcaac 721 agaccttgca ctcaactttg ctgaatccca ttccaaaatg tcggccagcg ctctgcacct 781 tactatctca tgacttcttc agaaatgatt ttctggaagt tgtgaatttc ttgaaaagtt 841 taacattgaa gagtgaagag gagaaaacgg aattctttaa atttctgctg gacagagtca 901 gctgcttgtc agaggaattg atagcttcaa ggttggtgcc tcttctgctt aatcagttgg 961 tgtttgcaga gccagtggct gttaagagtt ttcttcctta tctgcttggc cccaaaaaag 1021 atcatgcgca gggagaaact ccttgcttgc tctcaccagc cctgttccag tcacgggtga 1081 tccccgtgct tctccagttg tttgaagttc atgaagagca tgtgcggatg gtgctgctgt 1141 ctcacatcga ggcctacgtg gagcacttca ctcaggagca gctgaagaaa gtcatcttgc 1201 cacaggtttt gctgggcctg cgtgatacta gtgattccat tgtggcaatt actctgcata 1261 gcctagcagt gctggtctct ctgcttggac cagaggtggt tgtgggagga gaacgaacca 1321 agatcttcaa acgcactgcc ccaagtttta ctaaaaatac tgacctttct ctagaaggtg 1381 atccattttc tcagcctatt aaatttccca taaacggact ctcagatgta aaaaatactt 1441 cggaggacag tgaaaacttc ccatcaagtt ctaaaaagtc tgaggagtgg cctgactgga 1501 gtgaacctga ggagcctgaa aatcaaactg tcaacataca gatttggcct agagaacctt 1561 gtgatgatgt caagtcccag tgcactacct tggatgtgga agagtcatct tgggatgact 1621 gcgagcccag cagcttagat actaaagtaa acccaggagg tggaatcact gctacaaaac 1681 ctgttacctc agcggagcag aagcctattc cTGCTTTGCT TTCACTCACT GAAGAGTCTA 1741 TGCCTTGGAA ATCAAGCTTA CCCCAAAAGA TTAGCCTTGT ACAAAGGGGG GATGACGCAG 1801 ACCAAATCGA GCCGCCAAAA GTGTCATCAC AAGAAAGGCC CCTTAAGGTT CCATCAGAAC 1861 TTGGTTTAGG AGAGGAATTC ACCATTCAAG TAAAAAAGAA GCCAGTAAAA GATCCTGAGA 1921 TGGATTGGTT TGCTGATATG ATCCCAGAAA TTAAGCCTTC TGCTGCTTTT CTTATATTAC 1981 CTGAACTGAG GACAGAAATG GTCCCAAAAA AGGATGATGT CTCCCCAGTG ATGCAGTTTT 2041 CCTCAAAATT TGCTGCAGCA GAAATTACTG AGGGAGAGGC TGAAGGCTGG GAAGAAGAAG 2101 GGGAGCTGAA CTGGGAAGAT AATAACTGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2161 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2221 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAACT 2281 AATTTCGTCT TAACAAGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2341 tgaaagatt