Construct: ORF TRCN0000468901

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011779.2_s317c1
Derived from:
ccsbBroadEn_14920
DNA Barcode:
ACACAAGCGCTTCACCGCCTAGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS6KA4 (8986)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468901
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8986 RPS6KA4 ribosomal protein S6 kinase A4 XM_005274380.1 70.1% 66.8% (many diffs)
2 human 8986 RPS6KA4 ribosomal protein S6 kinase A4 NM_001300802.2 68.3% 68.3% (many diffs)
3 human 8986 RPS6KA4 ribosomal protein S6 kinase A4 NM_001006944.2 68.2% 68.2% (many diffs)
4 human 8986 RPS6KA4 ribosomal protein S6 kinase A4 NM_003942.3 67.7% 67.7% (many diffs)
5 human 8986 RPS6KA4 ribosomal protein S6 kinase A4 NM_001318361.2 59.5% 59.5% (many diffs)
6 mouse 56613 Rps6ka4 ribosomal protein S6 kinase... XM_017318262.1 63.5% 51.5% (many diffs)
7 mouse 56613 Rps6ka4 ribosomal protein S6 kinase... NM_019924.2 60% 65.2% (many diffs)
8 mouse 56613 Rps6ka4 ribosomal protein S6 kinase... XM_017318261.1 52.6% 57.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1638
ORF length:
1572
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ggacgaggac gacgatgaga gctgcgccgt ggagctgcgg atcacagaag
121ccaacctgac cgggcacgag gagaaggtga gcgtggagaa cttcgagctg ctcaaggtgc
181tgggcacggg agcctacggc aaggtgttcc tggtgcggaa ggcgggcggg cacgacgcgg
241ggaagctgta cgccatgaag gtgctgcgca aggcggcgct ggtgcagcgc gccaagacgc
301aggagcacac gcgcaccgag cgctcggtgc tggagctggt gcgccaggcg cccttcctgg
361tcacgctgca ctacgctttc cagacggatg ccaagctgca cctcatcctg gactatgtga
421gcggcgggga gatgttcacc cacctctacc agcgccagta cttcaaggag gctgaggtgc
481gcgtgtatgg gggtgagatc gtgctggccc tggaacacct gcacaagctc ggcatcattt
541accgagacct gaaactggag aatgtgctgc tggactccga gggccacatt gtcctcacgg
601acttcgggct gagcaaggag ttcctgacgg aggagaaaga gcggaccttc tccttctgtg
661gcaccatcga gtacatggcc cccgaaatca tccgtagcaa gacggggcat ggcaaggctg
721tggactggtg gagcctgggc atcttgctct tcgagctgct gacgggggcc tcgcccttca
781ccctggaggg cgagaggaac acgcaggctg aggtgtctcg acggatcctg aagtgctccc
841ctcccttccc ccctcggatc gggcccgtgg cgcaggacct gctgcagcgg ctgctttgta
901aggatcctaa gaagcgattg ggcgcggggc cccagggggc acaagaagtc cggaaccatc
961ccttcttcca gggcctcgat tgggtggctc tggctgccag gaagattcca gccccattcc
1021ggccccaaat ccgctcagag ctggatgtgg gcaactttgc ggaggaattc actcggctgg
1081agcctgtcta ctcaccccct ggcagccccc cacctgggga cccccgaatc tttcagggat
1141actcctttgt ggcaccctcc attctctttg accacaacaa cgcggccgag atcatgtgca
1201aaatccgcga ggggcgcttc tcccttgacg gGGAGGCCTG GCAGGGTGTA TCCGAGGAAG
1261CCAAGGAGCT GGTCCGAGGG CTCCTGACCG TGGACCCCGC CAAGCGGCTG AAGCTCGAGG
1321GACTGCGGGG CAGCTCGTGG CTGCAGGACG GCAGCGCGCG CTCCTCGCCC CCGCTCCGGA
1381CGCCCGACGT GCTCGAGTCC TCTGGGCCCG CAGTGCGCTC GGGTCTCAAC GCCACCTTCA
1441TGGCATTCAA CCGGGGCAAG CGGGAGGGCT TCTTCCTGAA GAGCGTGGAG AACGCACCCC
1501TGGCCAAGCG GCGGAAGCAG AAGCTGCGGA GCGCCACCGC CTCCCGCCGG GGCTCCCCTG
1561CACCAGCCAA CCCGGGCCGA GCCCCCGTCG CCGCCAAAGG GGCCCCCCGC CGAGCCAACG
1621GCCCCCTGCC CCCCTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1681TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1741GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACACAAG CGCTTCACCG
1801CCTAGGAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)