Transcript: Human NM_001318361.2

Homo sapiens ribosomal protein S6 kinase A4 (RPS6KA4), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
RPS6KA4 (8986)
Length:
3082
CDS:
218..2347

Additional Resources:

NCBI RefSeq record:
NM_001318361.2
NBCI Gene record:
RPS6KA4 (8986)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146216 CCAGCGCCAGTACTTCAAGG pXPR_003 AGG 211 10% 4 0.7224 RPS6KA4 RPS6KA4 77920
2 BRDN0001149050 CTTGCTACGGATGATTTCGG pXPR_003 GGG 430 20% 6 0.4352 RPS6KA4 RPS6KA4 77921
3 BRDN0001487122 CGCCACGGGCCCGATCCGAG pXPR_003 GGG 601 28% 8 0.0326 RPS6KA4 RPS6KA4 77919
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318361.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000021518 CGAAATCATCCGTAGCAAGAC pLKO.1 646 CDS 100% 4.050 5.670 N RPS6KA4 n/a
2 TRCN0000021514 CCAGACAGAGCAGAAGTATTT pLKO.1 2479 3UTR 100% 13.200 10.560 N RPS6KA4 n/a
3 TRCN0000021517 ACCCTCCATTCTCTTTGACCA pLKO.1 1117 CDS 100% 2.640 2.112 N RPS6KA4 n/a
4 TRCN0000199078 CGTCCACCAAAGACCCGTGTT pLKO.1 2786 3UTR 100% 1.350 1.080 N RPS6KA4 n/a
5 TRCN0000279791 CGTCCACCAAAGACCCGTGTT pLKO_005 2786 3UTR 100% 1.350 1.080 N RPS6KA4 n/a
6 TRCN0000199485 GCAGGGTGTATCCGAGGAAGC pLKO.1 1948 CDS 100% 0.000 0.000 N RPS6KA4 n/a
7 TRCN0000297323 GCAGGGTGTATCCGAGGAAGC pLKO_005 1948 CDS 100% 0.000 0.000 N RPS6KA4 n/a
8 TRCN0000021516 GCACAAGCTCGGCATCATTTA pLKO.1 484 CDS 100% 13.200 9.240 N RPS6KA4 n/a
9 TRCN0000279790 GCACAAGCTCGGCATCATTTA pLKO_005 484 CDS 100% 13.200 9.240 N RPS6KA4 n/a
10 TRCN0000199770 GCCACCTTCATGGCATTCAAC pLKO.1 2138 CDS 100% 4.950 3.465 N RPS6KA4 n/a
11 TRCN0000021515 CCGAAATCATCCGTAGCAAGA pLKO.1 645 CDS 100% 4.050 2.835 N RPS6KA4 n/a
12 TRCN0000195726 CCTCACTTTATGTCATCTGCT pLKO.1 2854 3UTR 100% 2.640 1.848 N RPS6KA4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318361.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489074 ATCCTCGGACATCTAGAGCCCTCG pLX_317 16.1% 91.7% 91.7% V5 (not translated due to prior stop codon) 0_1ins189;1096C>T n/a
2 ccsbBroadEn_11324 pDONR223 100% 59.5% 59.5% None (many diffs) n/a
3 ccsbBroad304_11324 pLX_304 0% 59.5% 59.5% V5 (many diffs) n/a
4 TRCN0000471622 TCCTTCTTACCGCACCACTCCGAC pLX_317 26.1% 59.5% 59.5% V5 (many diffs) n/a
5 ccsbBroadEn_14920 pDONR223 0% 59.5% 59.5% None (many diffs) n/a
6 ccsbBroad304_14920 pLX_304 0% 59.5% 59.5% V5 (many diffs) n/a
7 TRCN0000468901 ACACAAGCGCTTCACCGCCTAGGA pLX_317 26.1% 59.5% 59.5% V5 (many diffs) n/a
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