Construct: ORF TRCN0000468913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016663.2_s317c1
- Derived from:
- ccsbBroadEn_13831
- DNA Barcode:
- GAGGATCCGTACGAAATCCACCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CKM (1158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468913
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1158 | CKM | creatine kinase, M-type | NM_001824.5 | 99.6% | 98.9% | (many diffs) |
| 2 | mouse | 12715 | Ckm | creatine kinase, muscle | NM_007710.2 | 90.2% | 95.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc attcggtaac acccacaaca agttcaagct gaattacaag cctgaggagg 121 agtaccccga cctcagcaaa cataacaacc acatggccaa ggtactgacc cttgaactct 181 acaagaagct gcgggacaag gagactccat ctggcttcac tgtagacgat gtcatccaga 241 caggagtgga caacccaggt caccccttca tcatgaccgt gggctgcgtg gctggtgatg 301 aggagtccta cgaagttttc aaggaactct ttgaccccat catctcggat cgccacgggg 361 gctacaaacc cactgacaag cacaagactg acctcaacca tgaaaacctc aagggtggag 421 acgacctgga ccccaactac gtgctcagca gccgcgtccg cactggccgc agcatcaagg 481 gctacacgtt gcccccacac tgctcccgtg gcgagcgccg ggcggtggag aagctctctg 541 tggaagctct caacagcctg acgggcgagt tcaaagggaa gtactaccct ctgaagagca 601 tgacggagaa ggagcagcag cagctcatcg atgaccactt cctgttcgac aagcccgtgt 661 ccccgctgct gctggcctca ggcatggccc gcgactggcc cgacgcccgt ggcatctggc 721 acaatgacaa caagagcctc ctggtgtggg tgaacgagga ggatcacctc cgggtcatct 781 ccatggagaa ggggggcaac atgaaggagg ttttccgccg cttctgcgta gggctgcaga 841 agattgagga gaTCTTTAAG AAAGCTGGCC ACCCCTTCAT GTGGAACCAG CACCTGGGCT 901 ACGTGCTCAC CTGCCCATCC AACCTGGGCA CTGGGCTGCG TGGAGGCGTG CATGTGAAGC 961 TGGCGCACCT GAGCAAGCAC CCCAAGTTCG AGGAGATCCT CACCCGCCTG CGTCTGCAGA 1021 AGAGGGGTAC AGGTGGCGTG GACACAGCTG CCGTGGGCTC AGTATTTGAC GTGTCCAACG 1081 CTGATCGGCT GGGCTCGTCC GAAGTAGAAC AGGTGCAGCT GGTGGTGGAT GGTGTGAAGC 1141 TCATGGTGGA AATGGAGAAG AAGTTGGAGA AAGGCCAGTC CATCGACGAC ATGATCCCCG 1201 CCCCAGAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 1261 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 1321 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGGA TCCGTACGAA ATCCACCCAA 1381 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt