Construct: ORF TRCN0000468913

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016663.2_s317c1
Derived from:
ccsbBroadEn_13831
DNA Barcode:
GAGGATCCGTACGAAATCCACCCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKM (1158)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468913
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1158 CKM creatine kinase, M-type NM_001824.5 99.6% 98.9% (many diffs)
2 mouse 12715 Ckm creatine kinase, muscle NM_007710.2 90.2% 95.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc attcggtaac acccacaaca agttcaagct gaattacaag cctgaggagg
121agtaccccga cctcagcaaa cataacaacc acatggccaa ggtactgacc cttgaactct
181acaagaagct gcgggacaag gagactccat ctggcttcac tgtagacgat gtcatccaga
241caggagtgga caacccaggt caccccttca tcatgaccgt gggctgcgtg gctggtgatg
301aggagtccta cgaagttttc aaggaactct ttgaccccat catctcggat cgccacgggg
361gctacaaacc cactgacaag cacaagactg acctcaacca tgaaaacctc aagggtggag
421acgacctgga ccccaactac gtgctcagca gccgcgtccg cactggccgc agcatcaagg
481gctacacgtt gcccccacac tgctcccgtg gcgagcgccg ggcggtggag aagctctctg
541tggaagctct caacagcctg acgggcgagt tcaaagggaa gtactaccct ctgaagagca
601tgacggagaa ggagcagcag cagctcatcg atgaccactt cctgttcgac aagcccgtgt
661ccccgctgct gctggcctca ggcatggccc gcgactggcc cgacgcccgt ggcatctggc
721acaatgacaa caagagcctc ctggtgtggg tgaacgagga ggatcacctc cgggtcatct
781ccatggagaa ggggggcaac atgaaggagg ttttccgccg cttctgcgta gggctgcaga
841agattgagga gaTCTTTAAG AAAGCTGGCC ACCCCTTCAT GTGGAACCAG CACCTGGGCT
901ACGTGCTCAC CTGCCCATCC AACCTGGGCA CTGGGCTGCG TGGAGGCGTG CATGTGAAGC
961TGGCGCACCT GAGCAAGCAC CCCAAGTTCG AGGAGATCCT CACCCGCCTG CGTCTGCAGA
1021AGAGGGGTAC AGGTGGCGTG GACACAGCTG CCGTGGGCTC AGTATTTGAC GTGTCCAACG
1081CTGATCGGCT GGGCTCGTCC GAAGTAGAAC AGGTGCAGCT GGTGGTGGAT GGTGTGAAGC
1141TCATGGTGGA AATGGAGAAG AAGTTGGAGA AAGGCCAGTC CATCGACGAC ATGATCCCCG
1201CCCCAGAAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC
1261CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG
1321ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAGGA TCCGTACGAA ATCCACCCAA
1381CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)