Construct: ORF TRCN0000468915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018089.1_s317c1
- Derived from:
- ccsbBroadEn_07780
- DNA Barcode:
- TGAGCGAGTGCGAGCCATGCCTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLG2 (11232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11232 | POLG2 | DNA polymerase gamma 2, acc... | NM_007215.4 | 99.9% | 99.7% | 1247G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1521
- ORF length:
- 1455
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ctctcgtgta gccgtcaggg cctgccataa ggtctgcagg tgcctgttgt 121 ctgggtttgg gggtcgagta gatgcggggc agccggagct gttgacggaa aggagtagcc 181 ccaaaggagg gcatgtgaag tcgcacgcgg agctcgaggg gaacggcgag cacccagaag 241 cccccgggtc tggagaggga agcgaggcgc tgttagagat ctgtcagaga aggcatttcc 301 taagtggaag caagcagcag cttagccggg attctcttct gagtgggtgc caccccggct 361 tcggaccctt gggcgtagag ttgcggaaga acctggccgc agaatggtgg acctcggtgg 421 tggtgttcag ggagcaggta ttcccggtgg acgccctcca ccacaaacca ggccctttgc 481 tacccgggga cagtgccttc aggttagttt ctgcagaaac tctacgcgaa atcttgcaag 541 acaaagagct gagtaaggaa cagctagtag catttcttga gaacgtatta aaaacttctg 601 ggaaactacg ggagaacctt cttcacggtg ccttggaaca ctatgttaat tgcctggatc 661 tggtaaacaa gaggctacct tatggccttg ctcagattgg agtgtgtttt catcctgttt 721 ttgacactaa gcagatacga aatggtgtta aaagtattgg tgagaagact gaagcttcgt 781 tagtatggtt tactcctccg agaacttcaa accagtggct tgatttctgg ttacgtcatc 841 gactccagtg gtggagaaag tttgccatga gtccatctaa cttcagcagc agtgactgtc 901 aggatgaaga aggccggaaa ggaaacaaac tttactacaa ttttccctgg ggaaaggagt 961 taatagaaac cctgtggaac ctaggagatc acgaactttt acacatgtat cctggcaatg 1021 tgtctaaatt acatggccga gatggacgaa aaaatgtggt tccttgtgtt ctctctgtaa 1081 atggggacct agaccgaggc atgctggcct acctctatga ttctttccag ctgacagaga 1141 actcctttac aagaaagaaa aatcttcata gaaaggtact taaacttcac ccttgtttag 1201 cccctattaa ggttgctttG GATGTAGGAA GAGGCCCCAC ATTGGAACTA AGACAGGTTT 1261 GTCAAGGGCT ATTTAATGAG TTACTAGAAA ATGGGATTTC TGTGTGGCCT GCTTATTTGG 1321 AAACTATGCA GTCCTCATTG GAACAACTTT ATTCGAAGTA TGATGAAATG AGTATTCTCT 1381 TCACAGTTTT GGTTACTGAA ACTACTTTGG AGAATGGATT AATACATCTG AGAAGCAGAG 1441 ACACCACAAT GAAGGAAATG ATGCATATAT CCAAATTAAA AGACTTTTTG ATTAAGTATA 1501 TATCATCAGC TAAGAATGTA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1561 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1621 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGAG CGAGTGCGAG 1681 CCATGCCTCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt