Construct: ORF TRCN0000468915

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018089.1_s317c1
Derived from:
ccsbBroadEn_07780
DNA Barcode:
TGAGCGAGTGCGAGCCATGCCTCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POLG2 (11232)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468915
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11232 POLG2 DNA polymerase gamma 2, acc... NM_007215.4 99.9% 99.7% 1247G>C
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1521
ORF length:
1455
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg ctctcgtgta gccgtcaggg cctgccataa ggtctgcagg tgcctgttgt
121ctgggtttgg gggtcgagta gatgcggggc agccggagct gttgacggaa aggagtagcc
181ccaaaggagg gcatgtgaag tcgcacgcgg agctcgaggg gaacggcgag cacccagaag
241cccccgggtc tggagaggga agcgaggcgc tgttagagat ctgtcagaga aggcatttcc
301taagtggaag caagcagcag cttagccggg attctcttct gagtgggtgc caccccggct
361tcggaccctt gggcgtagag ttgcggaaga acctggccgc agaatggtgg acctcggtgg
421tggtgttcag ggagcaggta ttcccggtgg acgccctcca ccacaaacca ggccctttgc
481tacccgggga cagtgccttc aggttagttt ctgcagaaac tctacgcgaa atcttgcaag
541acaaagagct gagtaaggaa cagctagtag catttcttga gaacgtatta aaaacttctg
601ggaaactacg ggagaacctt cttcacggtg ccttggaaca ctatgttaat tgcctggatc
661tggtaaacaa gaggctacct tatggccttg ctcagattgg agtgtgtttt catcctgttt
721ttgacactaa gcagatacga aatggtgtta aaagtattgg tgagaagact gaagcttcgt
781tagtatggtt tactcctccg agaacttcaa accagtggct tgatttctgg ttacgtcatc
841gactccagtg gtggagaaag tttgccatga gtccatctaa cttcagcagc agtgactgtc
901aggatgaaga aggccggaaa ggaaacaaac tttactacaa ttttccctgg ggaaaggagt
961taatagaaac cctgtggaac ctaggagatc acgaactttt acacatgtat cctggcaatg
1021tgtctaaatt acatggccga gatggacgaa aaaatgtggt tccttgtgtt ctctctgtaa
1081atggggacct agaccgaggc atgctggcct acctctatga ttctttccag ctgacagaga
1141actcctttac aagaaagaaa aatcttcata gaaaggtact taaacttcac ccttgtttag
1201cccctattaa ggttgctttG GATGTAGGAA GAGGCCCCAC ATTGGAACTA AGACAGGTTT
1261GTCAAGGGCT ATTTAATGAG TTACTAGAAA ATGGGATTTC TGTGTGGCCT GCTTATTTGG
1321AAACTATGCA GTCCTCATTG GAACAACTTT ATTCGAAGTA TGATGAAATG AGTATTCTCT
1381TCACAGTTTT GGTTACTGAA ACTACTTTGG AGAATGGATT AATACATCTG AGAAGCAGAG
1441ACACCACAAT GAAGGAAATG ATGCATATAT CCAAATTAAA AGACTTTTTG ATTAAGTATA
1501TATCATCAGC TAAGAATGTA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1561CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1621AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGAG CGAGTGCGAG
1681CCATGCCTCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)