Construct: ORF TRCN0000468930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016695.1_s317c1
- Derived from:
- ccsbBroadEn_05305
- DNA Barcode:
- CATCTGCCAGATGTTGCAATTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TXNDC8 (255220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255220 | TXNDC8 | thioredoxin domain containi... | NM_001003936.4 | 100% | 100% | |
| 2 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014581.1 | 88.7% | 85% | (many diffs) |
| 3 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_011518463.2 | 82.6% | 75.4% | (many diffs) |
| 4 | human | 255220 | TXNDC8 | thioredoxin domain containi... | NM_001286946.2 | 82.6% | 82.6% | 129_130ins60 |
| 5 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014579.1 | 81.6% | 78.2% | (many diffs) |
| 6 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014583.1 | 80.8% | 70.9% | (many diffs) |
| 7 | human | 255220 | TXNDC8 | thioredoxin domain containi... | NM_001364963.1 | 75.2% | 69.3% | (many diffs) |
| 8 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014580.1 | 74.1% | 60.4% | (many diffs) |
| 9 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014582.1 | 67.1% | 63.7% | (many diffs) |
| 10 | human | 255220 | TXNDC8 | thioredoxin domain containi... | NM_001286947.1 | 59.6% | 46.5% | (many diffs) |
| 11 | human | 255220 | TXNDC8 | thioredoxin domain containi... | XM_017014584.1 | 54.8% | 51.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 414
- ORF length:
- 345
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGTACAGATT ATTAAAGACA CGAATGAATT TAAAACATTT TTGACAGCTG 121 CCGGACACAA ACTCGCAGTG GTTCAATTTT CTTCGAAACG GTGTGGTCCC TGCAAAAGGA 181 TGTTTCCTGT TTTCCATGCT ATGTCTGTGA AATACCAAAA TGTATTTTTT GCTAATGTGG 241 ATGTGAACAA TTCTCCGGAG CTGGCTGAAA CTTGTCACAT CAAAACAATA CCCACATTTC 301 AGATGTTCAA GAAAAGCCAG AAGGTAACCC TATTCTCAAG AATCAAAAGA ATAATTTGCT 361 GTTATAGAAG TGGATTCATG AGCAACCTGT GTCTTGCAGA TGATGGAAAT GAGTTGCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAC ATCTGCCAGA TGTTGCAATT GATACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att