Construct: ORF TRCN0000468932
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003838.1_s317c1
- Derived from:
- ccsbBroadEn_09745
- DNA Barcode:
- CATTACGGCACGGCCGCTTAAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF519 (162655)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468932
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162655 | ZNF519 | zinc finger protein 519 | NM_145287.4 | 99.8% | 99.6% | 499A>G;616A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1686
- ORF length:
- 1620
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga actcttaaca ttcagggatg tggccataga attctctcca gaagagtgga 121 aatgcctaga ccctgcccaa cagaatttat atagagatgt gatgttggag aactacagaa 181 acctcgtctc cctggctgtg tattcttatt acaaccaagg cattttacca gagcaaggca 241 tacaagattc attcaaaaaa gcaacactgg gaagatatgg gagctgtggc cttgaaaata 301 tatgcttatg gaaaaactgg gaaagtatag gtgaaggtga aggacaaaag gaatgttata 361 atctatgtag ccaatatttg acaactagtc ataacaaaca tttaactgtg aaaggagaca 421 aagaatatag aatatttcag aagaagcctc agtttctgtc agctgctcct acagaaccat 481 gtattcctat gaataaatat caacataaat ttttgaaatc tgtcttttgt aataaaaatc 541 agataaattt taaccatgac tcagatatta gtaaacatca tagtactcat tttctagaaa 601 actattacaa ttgtaatgaa tgtgaaaaag tattttacca atcctcaaag cttattttcc 661 ctgaaaatat ccatattcaa gaaaagcctt acaactctaa tgaatgtggt gaaacttctg 721 acccattctc aaagcttact caacatcaaa gaatttatat tggagagagc tcacaaagat 781 gtaataaaaa atgtataata gtctttagtc aatcacatct aaagggacat aagataatta 841 acactggaga aaaatcagtg aaatataaag aacgtggcaa agcttttacc aggggcttac 901 atcttggaca tcagaaaatt catactggag agaaacctta caaatgtaaa aaatgtgaca 961 aagcctttaa caagagttca caccttgctc aacatcagag aatccatact ggagagaagc 1021 ctttcaagtg taaggaatgt ggcaaagctt ttaacagagg ctcatacctt actcaacatc 1081 agagaatcca tactggagag agagctttca aatgtgaaga atgtggcaaa gcctttaaca 1141 gggggtcata ccttactcaa caccagagaa tccataccgg agagaaacct ttcagatgta 1201 aggaatgtgg caaagccttt aatagaagct catacgttac tcagcatcag agaatgcata 1261 ctggagagaa aCCTTTCAAG TGTAAGGAAT GTGGCAAAGC TTTTAACAGA GCTTCACACC 1321 TTACTCAACA TCAGAGAATC CATACTGGAG AGAAACACTT CAAATGTAAA GAATGTGGCA 1381 AAGCCTTTAA CAGGGGCTCA CACCTTACTC GACATCAAAG AATCCATACT GGAGAGAAGT 1441 CTTTCAAATG TGAAGAATGT GGCAAAGCTT TTATCTGGGG CTCACACCTT ACTCAACATC 1501 AGAGAGTCCA TACTGGAGAG AAATTCTTCA AATGTAAAGA ATGTGGCAAA GCTTTTACCA 1561 GGAGCTCACA CCTTACTCAA CATCAGAGAA TTCATACTGG AGAGAAACCT TTCAAATGTA 1621 AAGAATGTGG CAAAGCTTTT AACAGACGCT CAACCCTTAC TCAACATCAA ATAATTCATA 1681 CCAGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACATTACGGC ACGGCCGCTT AAGTGACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt