Construct: ORF TRCN0000468933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003279.1_s317c1
- Derived from:
- ccsbBroadEn_11033
- DNA Barcode:
- ATTACGCCTGGACTGGTGATTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLRG1 (5356)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5356 | PLRG1 | pleiotropic regulator 1 | NM_001201564.1 | 99.8% | 100% | 1005A>G;1191T>A |
| 2 | human | 5356 | PLRG1 | pleiotropic regulator 1 | NM_002669.4 | 98.1% | 98.2% | 286_312del;1032A>G;1218T>A |
| 3 | mouse | 53317 | Plrg1 | pleiotropic regulator 1 | NM_016784.3 | 87.2% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1581
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cgaggaggta cagaaacatt ctgtacacac ccttgtgttc aggtcgttga 121 agaggaccca tgacatgttt gtagctgata atggaaaacc tgtgccttta gatgaagaga 181 gtcacaaacg aaaaatggca atcaagcttc gtaatgagta tggtcctgtg ttgcatatgc 241 ctacttcaaa agaaaatctt aaagagaagg gtcctcagaa tgcaacggat tcatatgttc 301 ataaacagta ccctgccaat caaggacaag aagttgaata ctttgtggca ggggttgctt 361 tgacagcaga tactaagatc cagagaatgc caagtgaatc agctgcacag tccttagcgg 421 tggcattacc tttgcagacc aaggctgatg caaatcgtac tgcccctagt ggaagtgaat 481 accgacatcc tggggcttct gaccgtccac agcctacagc gatgaattca attgtcatgg 541 agactggcaa taccaagaac tctgcactga tggctaaaaa agcccctaca atgccaaaac 601 cccagtggca cccaccgtgg aaactctaca gggttatcag tgggcatctt ggctgggttc 661 gatgtattgc tgtggaacct ggaaatcagt ggtttgttac tggatctgct gacagaacta 721 taaagatctg ggacttggct agtggcaaat taaaactgtc attgactggg catattagta 781 ctgtgcgggg cgtgatagta agcacaagga gcccatatct gttctcttgt ggagaagaca 841 aacaagtgaa atgctgggat ctcgaataca ataaggttat acggcattat catggacatt 901 taagtgcagt gtatggtttg gatttgcacc cgacaatcga tgtgttggta acctgtagtc 961 gagattcaac tgcacggatt tgggatgtga gaactaaagc cagtgtacac acattatctg 1021 gacatacaaa tgcagttgct acagtgagat gtcaggctgc agaaccacag attattacag 1081 gaagccatga tactacaatt cgattatggg atctggtggc tggaaaaACA AGAGTGACAT 1141 TAACAAATCA CAAAAAATCA GTTAGGGCTG TGGTTTTACA TCCAAGACAT TACACATTTG 1201 CATCTGGTTC TCCAGATAAC ATAAAGCAGT GGAAATTCCC TGATGGAAGT TTCATACAAA 1261 ATCTTTCCGG TCATAATGCT ATTATTAACA CATTGACGGT AAATTCTGAT GGAGTGCTTG 1321 TATCTGGAGC TGACAATGGC ACCATGCATC TTTGGGACTG GAGAACTGGC TACAATTTTC 1381 AGAGAGTTCA CGCAGCTGTG CAACCTGGGT CTTTGGACAG TGAATCAGGA ATATTTGCTT 1441 GTGCTTTTGA TCAGTCTGAA AGTCGATTAC TAACAGCTGA AGCTGATAAA ACCATTAAAG 1501 TATACAGAGA GGATGACACA GCCACAGAAG AAACTCATCC AGTCAGCTGG AAACCAGAAA 1561 TTATCAAGAG AAAGAGATTT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1621 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1681 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATTA CGCCTGGACT 1741 GGTGATTTAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt