Construct: ORF TRCN0000468939
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001929.1_s317c1
- Derived from:
- ccsbBroadEn_07967
- DNA Barcode:
- CGAAAACCCGTTACCAGGAAGCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMHD1 (25939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468939
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25939 | SAMHD1 | SAM and HD domain containin... | NM_015474.3 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 25939 | SAMHD1 | SAM and HD domain containin... | NM_001363729.2 | 94.1% | 94% | (many diffs) |
| 3 | human | 25939 | SAMHD1 | SAM and HD domain containin... | NM_001363733.2 | 92.9% | 93.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1944
- ORF length:
- 1878
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcgagccgat tccgagcagc cctccaagcg tccccgttgc gatgacagcc 121 cgagaacccc ctcaaacacc ccttccgcag aggcagactg gtccccgggc ctggaactcc 181 atcccgacta caagacatgg ggtccggagc aggtgtgctc cttcctcagg cgcggtggct 241 ttgaagagcc ggtgctgctg aagaacatcc gagaaaatga aatcacaggc gcattactgc 301 cttgtcttga tgagtctcgt tttgaaaatc ttggagtaag ttccttgggg gagaggaaga 361 agctgcttag ttatatccag cgattggttc aaatccacgt tgatacaatg aaggtaatta 421 atgatcctat ccatggccac attgagctcc accctctcct cgtccgaatc attgatacac 481 ctcaatttca acgtcttcga tacatcaaac agctgggagg tggttactat gtttttccag 541 gagcttcgca caatcgattt gagcatagtc taggggtggg gtatctagca ggatgtctag 601 ttcacgcact gggtgaaaaa caaccagagc tgcagataag tgaacgagat gttctctgtg 661 ttcagattgc tggactttgt catgatctcg gtcatgggcc attttctcac atgtttgatg 721 gacgatttat tccacttgct cgcccggagg tgaaatggac gcatgaacaa ggctcagtta 781 tgatgtttga gcaccttatt aattctaatg gaattaagcc tgtcatggaa caatatggtc 841 tcatccctga agaagatatt tgctttataa aggaacaaat tgtaggacca cttgaatcac 901 ctgtagaaga ttcattgtgg ccatataaag ggcgtcctga aaacaaaagc ttcctttatg 961 agatagtatc taataaaaga aatggcattg atgtggacaa atgggattat tttgccaggg 1021 actgccatca tcttggaatc caaaataatt ttgattacaa gcgctttatt aagtttgccc 1081 gtgtctgtga agtagacaat gagttgcgta tttgtgctag agataaggaa gttggaaatc 1141 tgtatgacat gttccacact cgcaactctt tacaccgtag agcttatcaa cacaaagttg 1201 gcaacattat tgatacaatg attacagatg ctttcctcaa agcagatgac tacatagaga 1261 ttacaggtgc tggagaaaaa aagtatcgca tttctacagc aattgacgac atggaagcct 1321 atactaagct gacagataac atttttctgg agattttata ctctactgat cccaaattga 1381 aagacgcacg agagatttta aaacaaattg aataccgtaa tctattcaag tatgtgggtg 1441 agacgcagcc aacaggacaa ataaagatta aaaggGAGGA CTATGAATCT CTTCCAAAAG 1501 AGGTTGCCAG TGCTAAACCC AAAGTATTGC TAGACGTGAA ACTGAAGGCT GAAGATTTTA 1561 TAGTGGATGT TATCAACATG GATTATGGAA TGCAAGAAAA GAATCCAATT GATCATGTTA 1621 GCTTCTATTG TAAGACTGCC CCCAACAGAG CAATCAGGAT TACTAAAAAC CAGGTTTCAC 1681 AACTTCTGCC AGAGAAATTT GTAGAGCAGC TGATTCGAGT ATATTGTAAG AAGGTGGACA 1741 GAAAGAGTTT GTATGCCGCA AGACAATATT TTGTTCAGTG GTGTGCAGAC AGAAATTTCA 1801 CCAAGCCGCA GGATGGCGAT GTTATAGCCC CACTCATAAC ACCTCAAAAA AAGGAATGGA 1861 ACGACAGTAC TTCAGTCCAA AATCCAACTC GCCTCCGAGA AGCATCCAAA AGCAGAGTCC 1921 AGCTTTTTAA AGATGACCCA ATGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1981 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2041 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC GAAAACCCGT 2101 TACCAGGAAG CGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2161 att