Construct: ORF TRCN0000468945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016707.1_s317c1
- Derived from:
- ccsbBroadEn_15731
- DNA Barcode:
- AATAAACTGGAATCAAGCGCCTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SUB1 (10923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468945
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | NM_006713.4 | 99.4% | 5% | 12delA;31A>G |
| 2 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_011513944.3 | 99.4% | 5% | 12delA;31A>G |
| 3 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_017008986.1 | 99.4% | 5% | 12delA;31A>G |
| 4 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_017008987.1 | 99.4% | 5% | 12delA;31A>G |
| 5 | mouse | 20024 | Sub1 | SUB1 homolog (S. cerevisiae) | NM_011294.3 | 87.4% | 5% | (many diffs) |
| 6 | mouse | 20024 | Sub1 | SUB1 homolog (S. cerevisiae) | XM_006520042.3 | 87.4% | 5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 138
- ORF length:
- 72
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc TAAATCAAGG AACTTGTTTC TTCAGGCTCT TCTGGCAGTG ATTCTGACAG 121 TGAGGTTGAC AAAAAGTTAA AGAGGAAAAA GCAAGTTGCT CCAGAAAAAC CTGTAAAGAA 181 ACAAAAGACA GGTGAGACTT CGAGAGCCCT GTCATCTTCT AAACAGAGCA GCAGCAGCAG 241 AGATGATAAC ATGTTTCAGA TTGGGAAAAT GAGGTACGTT AGTGTTCGCG ATTTTAAAGG 301 CAAAGTGCTA ATTGATATTA GAGAATATTG GATGGATCCT GAAGGTGAAA TGAAACCAGG 361 AAGAAAAGGT ATTTCTTTAA ATCCAGAACA ATGGAGCCAG CTGAAGGAAC AGATTTCTGA 421 CATTGATGAT GCAGTAAGAA AACTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG 481 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 541 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAATAAACTG 601 GAATCAAGCG CCTCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 661 agatt