Construct: ORF TRCN0000468950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005579.1_s317c1
- Derived from:
- ccsbBroadEn_15566
- DNA Barcode:
- AACCCCGGAAACAGATACATTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGR (5995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5995 | RGR | retinal G protein coupled r... | XM_024448118.1 | 50.6% | 20.1% | (many diffs) |
| 2 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012722.1 | 42.5% | 17.1% | (many diffs) |
| 3 | human | 5995 | RGR | retinal G protein coupled r... | NM_002921.3 | 38.4% | 15.3% | (many diffs) |
| 4 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012720.2 | 37.1% | 14.9% | (many diffs) |
| 5 | human | 5995 | RGR | retinal G protein coupled r... | XR_002957005.1 | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaccagt gccttgccca ccggcttcgg ggagctcgag gtgctggctg 121 tggggatggt gctactggtg gaagaccccg gagctgcgGA CTCCCTGCCA CCTACTGGTG 181 CTGAGCTTGG CTCTTGCGGA CAGTGGGATC AGCCTGAATG CCCTCGTTGC AGCCACATCC 241 AGCCTTCTCC GTGTCTCCCA CAGGCGCTGG CCCTACGGCT CGGACGGCTG CCAGGCTCAC 301 GGCTTCCAGG GCTTTGTGAC AGCGTTGGCC AGCATCTGCA GCAGTGCAGC CATCGCATGG 361 GGGCGTTATC ACCACTACTG CACCCGCCAA AATGCTGCCA TCGGTCTCAT CATTTTGAGT 421 GGCGATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACGTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACCCCGGA AACAGATACA TTTTTACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt