Transcript: Human NM_001012720.2

Homo sapiens retinal G protein coupled receptor (RGR), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
RGR (5995)
Length:
2235
CDS:
39..914

Additional Resources:

NCBI RefSeq record:
NM_001012720.2
NBCI Gene record:
RGR (5995)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001012720.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358074 CGATCAATGCCATCAACTATG pLKO_005 814 CDS 100% 10.800 15.120 N RGR n/a
2 TRCN0000014250 CCTGTATCTATACGCAGTCAT pLKO.1 725 CDS 100% 4.950 6.930 N RGR n/a
3 TRCN0000014249 CCCTATGCCATCCTGTATCTA pLKO.1 714 CDS 100% 5.625 4.500 N RGR n/a
4 TRCN0000358075 AGTGGCCATCTCCAGGTAAAC pLKO_005 654 CDS 100% 10.800 7.560 N RGR n/a
5 TRCN0000358073 TATGCCATCCTGTATCTATAC pLKO_005 717 CDS 100% 10.800 7.560 N RGR n/a
6 TRCN0000014252 GCCCACGATCAATGCCATCAA pLKO.1 809 CDS 100% 4.950 3.465 N RGR n/a
7 TRCN0000358076 GTGTAACCATCACCCTAATAA pLKO_005 1238 3UTR 100% 15.000 9.000 N RGR n/a
8 TRCN0000014251 CTTCACCATGTCCTTCTTCAA pLKO.1 569 CDS 100% 4.950 2.970 N RGR n/a
9 TRCN0000014248 CCCACTTACAAGTGAGAACAT pLKO.1 1378 3UTR 100% 4.950 2.475 Y RGR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001012720.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06860 pDONR223 100% 98.3% 98.6% None (many diffs) n/a
2 ccsbBroad304_06860 pLX_304 0% 98.3% 98.6% V5 (many diffs) n/a
3 TRCN0000472982 TCACCCTCTAACTAAACGATACGA pLX_317 45.4% 98.3% 98.6% V5 (many diffs) n/a
4 TRCN0000488911 TCCCCCAAACGGTCCCGAGCAAAT pLX_317 37.3% 98.1% 98.3% V5 (many diffs) n/a
5 TRCN0000488892 GGCCCCTGCCTAATCCCCTTTAAG pLX_317 41.6% 98.3% 98.6% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_15566 pDONR223 0% 37.1% 14.9% None (many diffs) n/a
7 ccsbBroad304_15566 pLX_304 0% 37.1% 14.9% V5 (many diffs) n/a
8 TRCN0000468950 AACCCCGGAAACAGATACATTTTT pLX_317 73.1% 37.1% 14.9% V5 (many diffs) n/a
9 TRCN0000488317 GCCGCATCTAATGATAAATTTTTC pLX_317 91.5% 37.1% 14.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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