Construct: ORF TRCN0000468988
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005705.1_s317c1
- Derived from:
- ccsbBroadEn_03570
- DNA Barcode:
- CCCAGCACAAGAAGTGTACGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QRSL1 (55278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468988
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55278 | QRSL1 | glutaminyl-tRNA amidotransf... | NM_018292.5 | 100% | 100% | |
| 2 | human | 55278 | QRSL1 | glutaminyl-tRNA amidotransf... | XM_011535924.2 | 82.7% | 82.7% | 0_1ins273 |
| 3 | mouse | 76563 | Qrsl1 | glutaminyl-tRNA synthase (g... | NM_001081054.2 | 83.3% | 87.9% | (many diffs) |
| 4 | mouse | 76563 | Qrsl1 | glutaminyl-tRNA synthase (g... | XR_380090.1 | 61.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1650
- ORF length:
- 1584
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gggccggagc ctccgagaag tttctgcggc actgaaacaa ggccaaatta 121 caccaacaga gctctgtcaa aaatgtctct ctcttatcaa gaagaccaag tttctaaatg 181 cctacattac tgtgtcagaa gaggtggcct taaaacaagc tgaagaatca gaaaagagat 241 ataagaatgg acagtcactt ggggatttag atggaattcc tattgcagta aaagacaatt 301 tcagcacttc tggcattgag acaacatgtg catcaaatat gctgaaaggt tatataccac 361 cttataatgc tacagtagtt cagaagttgt tggatcaggg agctctacta atgggaaaaa 421 caaatttaga tgagtttgct atgggatctg ggagcacaga tggtgtattt ggaccagtta 481 aaaacccctg gagttattca aaacaatata gagaaaagag gaagcagaat ccccacagcg 541 agaatgaaga ttcagactgg ctgataactg gaggaagctc aggtgggagt gcagctgctg 601 tatcggcgtt cacatgctac gcggctttag gatcagatac aggaggatcg accagaaatc 661 ctgctgccca ctgtgggctt gttggtttca aaccaagcta tggcttagtt tcccgtcatg 721 gtctcattcc cctggtgaat tcgatggatg tgccaggaat cttaaccaga tgtgtggatg 781 atgcagcaat tgtgttgggt gcactggccg gacctgaccc cagggactct accacagtac 841 atgaacctat taataaacca ttcatgcttc ccagtttggc agatgtgagc aaactatgta 901 taggaattcc aaaggaatat cttgtaccgg aattatcaag tgaagtacag tctctttggt 961 ccaaagctgc tgacctcttt gagtctgagg gggccaaagt aattgaagta tcccttcctc 1021 acaccagtta ttcaattgtc tgctaccatg tattgtgcac atcagaagtg gcatcgaata 1081 tggcaagatt tgatgggcta caatatggtc acagatgtga cattgatgtg tccactgaag 1141 ccatgtatgc tgcaaccaga cgagaagggt ttaatgatgt gGTGAGAGGA AGAATTCTCT 1201 CAGGAAACTT TTTCTTATTA AAAGAAAACT ATGAAAATTA TTTTGTCAAA GCACAGAAAG 1261 TGAGACGCCT CATTGCTAAT GACTTTGTAA ATGCTTTTAA CTCTGGAGTA GATGTCTTGC 1321 TAACTCCCAC CACCTTGAGT GAGGCAGTAC CATACTTGGA GTTCATCAAA GAGGACAACA 1381 GAACCCGAAG TGCCCAGGAT GATATTTTTA CACAAGCTGT AAATATGGCA GGATTGCCAG 1441 CAGTGAGTAT CCCTGTTGCA CTCTCAAACC AAGGGTTGCC AATAGGACTG CAGTTTATTG 1501 GACGTGCGTT TTGTGACCAG CAGCTTCTTA CAGTAGCCAA ATGGTTTGAA AAACAAGTAC 1561 AGTTTCCTGT TATTCAACTT CAAGAACTCA TGGATGATTG TTCAGCAGTC CTTGAAAATG 1621 AAAAGTTAGC CTCTGTCTCT CTAAAACAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1681 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1741 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCCAG 1801 CACAAGAAGT GTACGATCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1861 tgaaagatt