Construct: ORF TRCN0000468997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009948.1_s317c1
- Derived from:
- ccsbBroadEn_07630
- DNA Barcode:
- CCGGACTTTCTTGATTTGATAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBD (10537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468997
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10537 | UBD | ubiquitin D | NM_006398.4 | 99.7% | 100% | 486T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcccaatgct tcctgcctct gtgtgcatgt ccgttccgag gaatgggatt 121 tAATGACCTT TGATGCCAAC CCATATGACA GCGTGAAAAA AATCAAAGAA CATGTCCGGT 181 CTAAGACCAA GGTTCCTGTG CAGGACCAGG TTCTTTTGCT GGGCTCCAAG ATCTTAAAGC 241 CACGGAGAAG CCTCTCATCT TATGGCATTG ACAAAGAGAA GACCATCCAC CTTACCCTGA 301 AAGTGGTGAA GCCCAGTGAT GAGGAGCTGC CCTTGTTTCT TGTGGAGTCA GGTGATGAGG 361 CAAAGAGGCA CCTCCTCCAG GTGCGAAGGT CCAGCTCAGT GGCACAAGTG AAAGCAATGA 421 TCGAGACTAA GACGGGTATA ATCCCTGAGA CCCAGATTGT GACTTGCAAT GGAAAGAGAC 481 TGGAAGATGG GAAGATGATG GCAGATTACG GCATCAGAAA GGGCAACTTA CTCTTCCTGG 541 CATGTTATTG CATTGGAGGG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCGG ACTTTCTTGA 721 TTTGATAGCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt