Construct: ORF TRCN0000469001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019025.2_s317c1
- Derived from:
- ccsbBroadEn_14626
- DNA Barcode:
- ACGCCCGCCAGGCTCCTGATCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA3 (2042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469001
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2042 | EPHA3 | EPH receptor A3 | NM_005233.6 | 99.9% | 100% | 2948_2949delTG |
| 2 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264715.3 | 99.8% | 99.8% | 1429_1430insAGC;2945_2946delTG |
| 3 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264716.3 | 93.4% | 93.1% | 2748_2749insG;2754_2754delAins193 |
| 4 | human | 2042 | EPHA3 | EPH receptor A3 | NM_182644.3 | 54.6% | 53.9% | (many diffs) |
| 5 | mouse | 13837 | Epha3 | Eph receptor A3 | NM_010140.3 | 88.7% | 96.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3018
- ORF length:
- 2949
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggattgtcag ctctccatcc tcctccttct cagctgctct gttctcgaca 121 gcttcgggga actgattccg cagccttcca atgaagtcaa tctactggat tcaaaaacaa 181 ttcaagggga gctgggctgg atctcttatc catcacatgg gtgggaagag atcagtggtg 241 tggatgaaca ttacacaccc atcaggactt accaggtgtg caatgtcatg gaccacagtc 301 aaaacaattg gctgagaaca aactgggtcc ccaggaactc agctcagaag atttatgtgg 361 agctcaagtt cactctacga gactgcaata gcattccatt ggttttagga acttgcaagg 421 agacattcaa cctgtactac atggagtctg atgatgatca tggggtgaaa tttcgagagc 481 atcagtttac aaagattgac accattgcag ctgatgaaag tttcactcaa atggatcttg 541 gggaccgtat tctgaagctc aacactgaga ttagagaagt aggtcctgtc aacaagaagg 601 gattttattt ggcatttcaa gatgttggtg cttgtgttgc cttggtgtct gtgagagtat 661 acttcaaaaa gtgcccattt acagtgaaga atctggctat gtttccagac acggtaccca 721 tggactccca gtccctggtg gaggttagag ggtcttgtgt caacaattct aaggaggaag 781 atcctccaag gatgtactgc agtacagaag gcgaatggct tgtacccatt ggcaagtgtt 841 cctgcaatgc tggctatgaa gaaagaggtt ttatgtgcca agcttgtcga ccaggtttct 901 acaaggcatt ggatggtaat atgaagtgtg ctaagtgccc gcctcacagt tctactcagg 961 aagatggttc aatgaactgc aggtgtgaga ataattactt ccgggcagac aaagaccctc 1021 catccatggc ttgtacccga cctccatctt caccaagaaa tgttatctct aatataaacg 1081 agacctcagt tatcctggac tggagttggc ccctggacac aggaggccgg aaagatgtta 1141 ccttcaacat catatgtaaa aaatgtgggt ggaatataaa acagtgtgag ccatgcagcc 1201 caaatgtccg cttcctccct cgacagtttg gactcaccaa caccacggtg acagtgacag 1261 accttctggc acatactaac tacacctttg agattgatgc cgttaatggg gtgtcagagc 1321 tgagctcccc accaagacag tttgctgcgg tcagcatcac aactaatcag gctgctccat 1381 cacctgtcct gacgattaag aaagatcgga cctccagaaa tagcatctct ttgtcctggc 1441 aagaacctga acatcctaat gggatcatat tggactacga ggtcaaatac tatgaaaagc 1501 aggaacaaga aacaagttat accattctga gggcaagagg cacaaatgtt accatcagta 1561 gcctcaagcc tgacactata tacgtattcc aaatccgagc ccgaacagcc gctggatatg 1621 ggacgaacag ccgcaagttt gagtttgaaa ctagtccaga ctctttctcc atctctggtg 1681 aaagtagcca agtggtcatg atcgccattt cagcggcagt agcaattatt ctcctcactg 1741 ttgtcatcta tgttttgatt gggaggttct gtggctataa gtcaaaacat ggggcagatg 1801 aaaaaagact tcattttggc aatgggcatt taaaacttcc aggtctcagg acttatgttg 1861 acccacatac atatgaagac cctacccaag ctgttcatga gtttgccaag gaattggatg 1921 ccaccaacat atccattgat aaagttgttg gagcaggtga atttggagag gtgtgcagtg 1981 gtcgcttaaa acttccttca aaaaaagaga tttcagtggc cattaagacc ctgaaagttg 2041 gctacacaga aaagcagagg agagacttcc tgggagaagc aagcattatg ggacagtttg 2101 accaccccaa tatcattcga ctggaaggag ttgttaccaa aagtaagcca gttatgattg 2161 tcacagaata catggagaat ggttccttgg atagtttcct acgtaaacac gatgcccagt 2221 ttactgtcat tcagctagtg gggatgcttc gagggatagc atctggcatg aagtacctgt 2281 cagacatggg ctatgttcac cgagacctcg ctgctcggaa catcttgatc aacagtaact 2341 tggtgtgtaa ggtttctgat ttcggacttt cgcgtgtcct ggaggatgac ccagaagctg 2401 cttatacaac aagaggaggg aagatcccaa tcaggtggac atcaccagaa gctatagcct 2461 accgcaagtt cacgtcagcc agcgatgtat ggagttatgg gattgttctc tgggaggtga 2521 tgtcttatgg agagagacca tactgggaga tgtccaatca ggatgtaatt aaagCTGTAG 2581 ATGAGGGCTA TCGACTGCCA CCCCCCATGG ACTGCCCAGC TGCCTTGTAT CAGCTGATGC 2641 TGGACTGCTG GCAGAAAGAC AGGAACAACA GACCCAAGTT TGAGCAGATT GTTAGTATTC 2701 TGGACAAGCT TATCCGGAAT CCCGGCAGCC TGAAGATCAT CACCAGTGCA GCCGCAAGGC 2761 CATCAAACCT TCTTCTGGAC CAAAGCAATG TGGATATCAC TACCTTCCGC ACAACAGGTG 2821 ACTGGCTTAA TGGTGTCTGG ACAGCACACT GCAAGGAAAT CTTCACGGGT GTGGAGTACA 2881 GTTCTTGTGA CACAATAGCC AAGATTTCCA CAGATGACAT GAAAAAGGTT GGTGTCACCG 2941 TGGTTGGGCC ACAGAAGAAG ATCATCAGTA GCATTAAAGC TCTAGAAACG CAATCAAAGA 3001 ATGGCCCAGT TCCCGTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3061 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3121 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACGCCCGCCA GGCTCCTGAT 3181 CCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt