Construct: ORF TRCN0000469017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016768.1_s317c1
- Derived from:
- ccsbBroadEn_02056
- DNA Barcode:
- ATCCTTGAAAAAGATCTAACTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRNA6 (8973)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8973 | CHRNA6 | cholinergic receptor nicoti... | NM_004198.3 | 100% | 100% | |
| 2 | human | 8973 | CHRNA6 | cholinergic receptor nicoti... | NM_001199279.1 | 96.9% | 96.9% | 219_220ins45 |
| 3 | mouse | 11440 | Chrna6 | cholinergic receptor, nicot... | NM_021369.2 | 81.4% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1548
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaccagcaag gggcagggat tccttcatgg gggcttgtgt ctctggctgt 121 gtgtgttcac acctttcttt aaaggctgtg tgggctgtgc aactgaggag aggctcttcc 181 acaaactgtt ttctcattac aaccagttca tcaggcctgt ggaaaacgtt tccgaccctg 241 tcacggtaca ctttgaagtg gccatcaccc agctggccaa cgtggatgaa gtaaaccaga 301 tcatggaaac caatttgtgg ctgcgtcaca tctggaatga ttataaattg cgctgggatc 361 caatggaata tgatggcatt gagactcttc gcgttcctgc agataagatt tggaagcccg 421 acattgttct ctataacaat gctgttggtg acttccaagt agaaggcaaa acaaaagctc 481 ttcttaaata caatggcatg ataacctgga ctccaccagc tatttttaag agttcctgcc 541 ctatggatat cacctttttc ccttttgatc atcaaaactg ttccctaaaa tttggttcct 601 ggacgtatga caaagctgaa attgatcttc taatcattgg atcaaaagtg gatatgaatg 661 atttttggga aaacagtgaa tgggaaatca ttgatgcctc tggctacaaa catgacatca 721 aatacaactg ttgtgaagag atatacacag atataaccta ttctttctac attagaagat 781 tgccgatgtt ttacacgatt aatctgatca tcccttgtct ctttatttca tttctaaccg 841 tgttggtctt ttaccttcct tcggactgtg gtgaaaaagt gacgctttgt atttcagtcc 901 tgctttctct gactgtgttt ttgctggtca tcacagaaac catcccatcc acatctctgg 961 tggtcccact ggtgggtgag tacctgctgt tcaccatgat ctttgtcaca ctgtccatcg 1021 tggtgactgt gtttgtgttg aacatacact accgcacccc aaccacgcac acaatgccca 1081 ggtgggtgaa gacagttttc ctgaagctgc tgccccaggt cctgctgatg aggtggcctc 1141 tggacaagac aaggggcaca ggctcTGATG CAGTGCCCAG AGGCCTTGCC AGGAGGCCTG 1201 CCAAAGGCAA GCTTGCAAGC CATGGGGAAC CCAGACATCT TAAAGAATGC TTCCATTGTC 1261 ACAAATCAAA TGAGCTTGCC ACAAGCAAGA GAAGATTAAG TCATCAGCCA TTACAGTGGG 1321 TGGTGGAAAA TTCGGAGCAC TCGCCTGAAG TTGAAGATGT GATTAACAGT GTTCAGTTCA 1381 TAGCAGAAAA CATGAAGAGC CACAATGAAA CCAAGGAGGT AGAAGATGAC TGGAAATACG 1441 TGGCCATGGT GGTGGACAGA GTATTTCTTT GGGTATTTAT AATTGTCTGT GTATTTGGAA 1501 CTGCAGGGCT ATTTCTACAG CCACTACTTG GGAACACAGG AAAATCTTAC CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGAATCCTTG AAAAAGATCT AACTGGCACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt